×

Assay procedures

  • US 4,804,625 A
  • Filed: 09/27/1984
  • Issued: 02/14/1989
  • Est. Priority Date: 09/27/1984
  • Status: Expired due to Fees
First Claim
Patent Images

1. A method for determining the presence of an analyte in a sample comprising contacting a sample with a reagent adapted to form a substrate phase and a product phase with said sample, said reagent including an enzyme-substrate pair, including an enzyme and a substrate, and labeled material, capable of assuming a bound position with said analyte, said labeled material having a label moiety capable of participating in reactions related to the presence of said analyte which reactions require said substrate present in said substrate phase and produce a reaction product, said label moiety is a member of said enzyme-substrate pair, said enzyme catalyzing a reaction with said substrate which produces said reaction product, said enzyme-substrate pair is selected from the group of enzyme-substrate pairs consisting of alkaline phosphatase and the substrate p-phenylazophenyl phosphate, and the enzyme collagenase and the substrate collagen/blue dextran, said reaction product capable of partitioning into said product phase distinct from said substrate phase wherein said substrate phase is a solid or a liquid, and said product phase is a liquid;

  • and monitoring said product phase for the presence of said reaction product.

View all claims
  • 2 Assignments
Timeline View
Assignment View
    ×
    ×