Dry enzyme formulations containing D-amino acid oxidase

• US 4,806,478 A
• Filed: 10/17/1984
• Issued: 02/21/1989
• Est. Priority Date: 10/17/1984
• Status: Expired due to Term

• Current Assignee: Minnesota Mining and Manufacturing Co (3M Company)
• Original Assignee: Minnesota Mining and Manufacturing Co (3M Company)
• Inventors: Stahl, Julie B.
• Primary Examiner(s): Warren, Charles F.
• Assistant Examiner(s): Jay, Jeremy M.
• Application Number:
  US06/661,662
• Time in Patent Office: 1,588 Days
• Field of Search: 435/18, 435/23-25, 435/28, 435/29, 435/32, 435/180, 435/188, 435/189, 435/805, 435/810, 422/55, 422/56, 422/74
• US Class Current: 435/180
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: C12Q 1/37 involving peptidase or proteinase
• CPC Class Codes: G01N 2333/906 acting on nitrogen containing compounds as donors (1.4, 1.5, 1.7)
• CPC Class Codes: G01N 33/521 Single-layer analytical elements

Abstract

A stable formulation of a non-cellulosic fabric having D-amino acid oxidase coated and dried thereon is shown. The fabric may be woven, nonwoven, or knit and may be made from polyolefin, nylon, polyester, or polyurethane fabrics. Polyolefin fabrics may be pretreated with a surfactant. Also shown are formulations containing some or all of the reagents used to detect beta lactam antibiotics. Kits having webs coated with analytically effective amounts of D-amino acid oxidase, peroxidase, flavin adenine, dinucleotide, a peroxide sensitive dye and a D,D-carboxypeptidase substrate containing a carboxyterminal D-alanine are shown. D,D-carboxypeptidase R39 enzyme may be provided separately in either aqueous form or dried on a separate piece of fabric. Under appropriate conditions the D,D-carboxypeptidase may also be dried on the fabric.

Claims

1. A stable enzyme formulation comprised of a non-cellulosic fibrous carrier having coated and dried thereon D-amino acid oxidase, wherein said enzyme is substantially solubilized when said formulation is rehydrated.

2. The formulation of claim 1 wherein the non-cellulosic fibrous carrier is selected from a group consisting of polyolefins, nylons, polyesters, and polyurethanes.

3. The formulation of claim 1 wherein the fibrous carrier is a nonwoven fibrous web.

4. The formulation of claim 2 wherein the fibrous carrier is a non-woven web.

5. The formulation of claim 1 wherein the fibrous carrier is a blown microfibrous web.

6. The formulation of claim 2 wherein the fibrous carrier is a blown microfibrous web.

7. The formulation of claim 2 wherein the fibrous carrier is a polybutylene blown microfibrous web and has been pretreated with surfactant.

8. The formulation of claim 2 wherein the fibrous carrier is a polypropylene blown microfibrous web and has been pretreated with surfactant.

9. A stable enzyme formulation prepared by coating and drying D-amino acid oxidase on a non-cellulosic fibrous carrier, wherein said enzyme is substantially solubilized when said formulation is rehydrated.