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Method of detecting nucleic acid sequences

  • US 4,808,519 A
  • Filed: 11/22/1985
  • Issued: 02/28/1989
  • Est. Priority Date: 04/06/1984
  • Status: Expired due to Term
First Claim
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1. A probe-vector molecule used for detecting a target nucleic acid containing a particular base sequence X1 X2 X3 . . . Xm . . . Y1 Y2 Y3 . . . Yn, said probe-vector comprisinga linear partially single stranded DNA molecule comprised of two strands, a long strand containing the sequence X'"'"'m X'"'"'m-1 . . . X'"'"'3 X'"'"'2 X'"'"'1 . . . Z'"'"'1 Z'"'"'2 Z'"'"'3 . . . Z'"'"'hd p . . . Y'"'"'n Y'"'"'n-1 . . . Y'"'"'3 Y'"'"'2 Y'"'"'1, and a short strand containing the sequence Z1 Z2 Z3 . . . Zp, wherein m, n, p and k are integers, and in whichfor any k, X'"'"'k is the base complementary to Xk is the base complementary to Yk, and Z'"'"'k is the base complementary to ZK, the ends of said long strand of said probe-vector being substantially complementary to sections of said target nucleic acid,m and n are sufficiently large so that when said probe-vector is added to said target nucleic acid under hybridizing conditions, stable hybridization will occur between the ends of said long strand of said probe-vector and the said substantially complementary section of said target nucleic acid forming thereby a circular hybrid, the probe-vector being circularized if the particular target sequence is present and has hybridized to said probe-vector, andthe regions Z1 Z2 Z3 . . . Zp and Z'"'"'1 Z'"'"'2 Z'"'"'3 . . . Z'"'"'p of said probe-vector strands contain a replicon and confer a detectable phenotype such that bacteria transformed by said hybrid are detectable and distinguishable from untransformed bacteria by detection of said phenotype.

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