Enzymes immobilized on polyamides or cellulose hydrate

• US 4,808,529 A
• Filed: 02/24/1986
• Issued: 02/28/1989
• Est. Priority Date: 03/13/1985
• Status: Expired due to Fees

• Current Assignee: Miles, Inc. (Bayer AG)
• Original Assignee: Miles, Inc. (Bayer AG)
• Inventors: Schranz, Karl-Wilhelm, Doppelfeld, Peter, Riebel, Alexander, Oosta, Gary
• Primary Examiner(s): Naff, David M.
• Application Number:
  US06/832,230
• Time in Patent Office: 1,100 Days
• Field of Search: 435/174, 435/177, 435/179, 435/180, 435/182, 435/288, 435/14, 435/25, 435/26, 435/28
• US Class Current: 435/179
• CPC Class Codes: B01D 15/08 Selective adsorption, e.g. chromatography
• CPC Class Codes: B01D 15/3804 Affinity chromatography
• CPC Class Codes: C12N 11/096 Polyesters; Polyamides
• CPC Class Codes: C12N 11/12 Cellulose or derivatives thereof
• CPC Class Codes: G01N 33/521 Single-layer analytical elements
• CPC Class Codes: G01N 33/548 Carbohydrates, e.g. dextran

Abstract

Proteins such as enzymes are attached directly to a polyamide or cellulose hydrate carrier. Attachment can be carried out by impregnation of the carrier with a protein solution. The carrier can be in the form of a membrane, and the polyamide can be nylon. Resulting carrier-bound proteins find utility as biocatalyst, as test strips or as chromatography materials. In a preferred embodiment, a polyamide or cellulose hydrate membrane carrier is impregnated with an enzyme selected from peroxidase, glucose oxidase, diaphorase and glucose dehydrogenase and then dried, coated with a luminol solution and again dried. The resultant membrane can be used for glucose determination by measuring chemiluminescence produced when the membrane contacts a glucose solution.

Claims

1. The process for forming a carrier-bound protein which comprises

impregnating a membrane carrier of polyamide or cellulose hydrate having functional groups from the group consisting of carboxyl groups, amino groups, sulphonic acid groups, imino groups, thio groups, hydroxyl groups, pyridyl groups, and phosphoryl groups with an enzyme from the group consisting of peroxidase, glucose oxidase, diaphorase and glucose dehydrogenase,

drying the impregnated carrier,

coating the resulting dried carrier with a luminol solution having an adjusted pH value of 9.7, and

then drying the luminol coated carrier.