Method and apparatus for monitoring blood components

• US 4,810,090 A
• Filed: 08/24/1987
• Issued: 03/07/1989
• Est. Priority Date: 08/24/1987
• Status: Expired due to Term

• Current Assignee: Gambro BCT, Inc. (Terumo Corporation)
• Original Assignee: COBE Laboratories Incorporated (Terumo Corporation)
• Inventors: Holmes, Brian M., Boucher, Terry D.
• Primary Examiner(s): McGraw, Vincent P.
• Application Number:
  US07/088,686
• Time in Patent Office: 561 Days
• Field of Search: 356/39, 356/40, 356/41, 356/73, 356/244, 356/410, 356/440, 250/576
• US Class Current: 356/39
• CPC Class Codes: G01N 2015/018 Platelets
• CPC Class Codes: G01N 2015/045 by optical analysis
• CPC Class Codes: G01N 2021/3181 using LEDs
• CPC Class Codes: G01N 2021/4711 Multiangle measurement
• CPC Class Codes: G01N 21/532 with measurement of scattering and transmission

Abstract

Monitoring concentration of components of blood flowing through a flowpath including flowing the components through a transparent channel, directing light to the channel along an axis intersecting the channel, detecting light passing through the channel along the axis, detecting light scattered off of the axis, and determining concentration of components based on the light passing along the axis and/or the light scattered off of the axis. Also disclosed is a movable cover to block ambient light from a photodetector after mounting of a removable transparent flow channel.

Claims

1. A method of monitoring concentration of components of blood flowing in a flowpath comprising

flowing said components through a transparent channel,

directing light to said channel along an axis intersecting said channel,

detecting light passing through said channel along said axis with a central detector,

detecting light scattered off of said axis with an annular detector, and

determining condition of components based on said light passing along said axis and/or said light scattered off said axis,

said determining involving using said central detector to determine concentration in a low range of concentration values and said annular detector to determine concentration in a high range of concentration values.

2. The method of claim 1 wherein said determining involves using said central detector to determine concentration below a point of inflection of an irradiance versus concentration function of light sensed by the annular detector and using the annular detector to determine concentration above two times said point of inflection.

3. The method of claim 1 wherein said determining also involves determining the ratio of light detected by said central detector and said annular detector to provide an index of scatter.

4. The method of claim 1 wherein said detectors are photodetectors providing signals, the magnitudes of said signals being functions of the detected light, and normalized irradiance values are employed in said determining.

5. The method of claim 4 wherein said directing light involves pulsing a light source on and off, and said normalized irradiance values are based on the difference in the magnitudes of said signals between on and off conditions.

6. The method of claim 5 wherein said detecting involves sampling the signals at a frequency that is a multiple of the ambient light frequency.

7. The method of claim 1 wherein said directing light includes directing light of different frequencies.

8. The method of claim 1 wherein said transparent channel is a plastic cuvette of a disposable tubing set.

9. The method of claim 8 further comprising separating blood from a patient/donor into separated fractions and directing one fraction to said transparent channel.

10. The method of claim 9 wherein said fraction comprises platelets and further comprising collecting said platelets after they have passed through said transparent channel.

11. The method of claim 8 further comprising providing a movable cover, and blocking ambient light from said channel and detectors with said cover during said directing and detecting.

12. Apparatus for monitoring concentration of components of blood flowing in a flowpath comprising

a transparent channel,

a source of light positioned to direct light to said channel along an axis intersecting said channel,

a central light detector positioned to detect said light passing through said channel along said axis and provide a central signal indicating said passing light,

an annular detector positioned to detect said light scattering off of said axis and provide an annular signal indicating said scattered light, and

a calculator connected to receive said central signal and said annular signal from said central and annular detectors and to determine conditions of components based upon said central signal and/or said annular signal,

said calculator using said central signal to determine concentration in a low range of concentration values and said annular signal to determine concentration in a high range of concentration values.

13. The apparatus of claim 12 wherein said calculator uses said central signal to determine concentration below a point of inflection of an irradiance versus concentration function of light sensed by the annular detector and uses the annular detector to determine concentration above two times said point of inflection.

14. The apparatus of claim 12 wherein said calculator determines the ratio of light detected by said central detector and said annular detector to provide an index of scatter.

15. The apparatus of claim 12 wherein said detectors are photodetectors, and normalized irradiance values are employed by said calculator.

16. The apparatus of claim 15 wherein said source of light is pulsed on and off, and said normalized irradiance values are based on the difference in the magnitudes of said signals between on and off conditions.

17. The apparatus of claim 16 wherein said signals of said detectors are sampled at a frequency that is a multiple of the ambient light frequency.

18. The apparatus of claim 12 wherein said source of light provides light of different frequencies.

19. The apparatus of claim 12 wherein said transparent channel is a plastic cuvette of a disposable tubing set.

20. The apparatus of claim 19 further comprising a blood separating centrifuge for separating blood from a patient/donor into separated fractions, and a tube directing one fraction to said transparent channel.

21. The apparatus of claim 20 wherein said fraction comprises platelets, and further comprising a platelet collection bag for collecting said platelets after they have passed through said transparent channel.

22. The apparatus of claim 19 further comprising a movable cover for blocking ambient light from said channel and detectors.

23. Apparatus for monitoring concentration of components of blood flowing in a flowpath comprising

a removable transparent channel comprising a rigid plastic cuvette made of optically clear transparent material permanently connected to flexible plastic tubing of a disposable tube set,

a channel engager having a recess for removably receiving said channel,

a source of light positioned to direct light to said plastic cuvette along an axis intersecting said plastic cuvette when mounted in said recess,

a light detector positioned to detect said light passing through said plastic cuvette when mounted in said recess, and

a movable cover movable between an open position in which said plastic cuvette can be placed in said recess and a closed position in which ambient light is blocked from said detector via said cover, said cover enclosing said cuvette within it when in said closed position,

said cover including means for guiding said cuvette into a predetermined position aligned with said source of light and said detector as said cover is moved to said closed position and means for holding said cuvette in said position.

24. The apparatus of claim 23 wherein said cover is slidably mounted along said axis.

25. The apparatus of claim 24 wherein said means for guiding and said means for holding comprise notches for receiving circuular extension portions of said cuvette to accurately locate said cuvette with respect to said axis, said notches converging along axes parallel to said axes to guide portions of said plastic cuvette into position.

26. The apparatus of claim 25 wherein said plastic cuvette has flat walls normal to said axis.

27. The apparatus of claim 23 wherein said cuvette has a smooth transition form a circular flowpath of said tube to a rectangular flowpath of said cuvette.