Plate reader

• US 4,810,096 A
• Filed: 04/08/1987
• Issued: 03/07/1989
• Est. Priority Date: 05/09/1986
• Status: Expired due to Fees

• Current Assignee: Cambridge Life Sciences PLC
• Original Assignee: Cambridge Life Sciences PLC
• Inventors: Russell, Andrew J., Calvert, Colin
• Primary Examiner(s): Rosenberger, Richard A.
• Application Number:
  US07/036,006
• Time in Patent Office: 699 Days
• Field of Search: 362/29, 362/23, 362/800, 356/436, 356/440, 356/443, 356/444
• US Class Current: 356/436
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 2201/0446 Multicell plate, sequential

Abstract

A small, compact plate reader is disclosed for microtitre plates. The reader comprises a totally enclosed housing (19), means e.g. a drawer (2) for receiving and inserting into the housing a microtitre plate (7) which is received into the housing between a light source and a photodetector positioned in vertical alignment above and below the microtitre plate. The light source and detector are mounted on a carriage movable in the housing along each of two orthogonal axes by means of the manual control knobs (10,11) on either side of the housing. The light source and detector are thus positionable with each of the wells of the microtitre plate in turn enabling absorbancy measurements to be made, and visually displayed by a digital display (9). A window (8) in the housing (1) marked with indices corresponding to the cells in the microtitre plate permits a visual indication of the position of the detector head relative to the microtitre plate, for example, by illumination from below of the appropriate index mark by the light source itself, or by a secondary light source.

Claims

1. A plate reader for microtitre plates, comprising a totally enclosed housing, plate supporting means within said housing for receiving and supporting in a horizontal position a microtitre plate containing in its wells samples the absorbance of which is to be measured by the plate reader, means in said housing providing access to said plate supporting means for the placement thereon and removal therefrom of said microtitre plate, a carriage movably mounted in the housing and carrying in spaced vertical alignment a light source and a light detector, said source and detector being positioned one above and one below the plane of the microtitre plate when in position on said support means, means for moving the carriage within the housing to as to selectively position said source and said detector in vertical alignment in turn with each of the wells of said microtitre plate, each of said wells in turn being positioned between the light source and the detector, indicator means attached to the carriage and visible externally of the housing to indicate the positioning of the light source and the detector relative to the wells of the microtitre plate, means for measuring the amount of light transmitted from the light source to the detector through each of the wells in turn, and means for indicating and/or recording the absorbance of the samples contained within those wells.

2. A plate reader according to claim 1, wherein the carriage is constrained within the housing to move along two axes at right angles at one to the other, and means are provided to move the carriage independently along each of those axes thereby to bring the light source and detector into vertical alignment in turn with each of the wells of the microtiter plate.

3. A plate reader according to claim 2, wherein said carriage is constructed in two parts, one constrained to move within said housing along one of said axes, and the other being movably mounted on the first and constrained to move thereonalong the other of said two axes.

4. A plate reader according to claim 1, wherein the means for providing access to the interior of the housing for the placement and removal of the microtitre plate comprise a drawer slidably mounted in the housing and wherein is located said plate supporting means whereby, when the drawer is open the microtitre plate can be placed therein or removed, and when the drawer is closed the plate is supported in a horizontal plane between the light source and the light detector.

5. A plate reader according to claim 3, wherein biassed detent means are provided to locate the carriage positively at each position within the housing whereat the said light source and detector are in vertical alignment with each in turn of said wells of said microtitre plate.

6. A plate reader according to claim 1, wherein said indicator means comprise a translucent window in the housing, through which a position indicating means attached to the carrier is visible thereby to indicate the position of the light source and the detector relative to the wells in the microtitre plate.

7. A plate reader according to claim 6, wherein the position indicating means is the light source itself.

8. A plate reader according to claim 1, wherein the plate supporting means, the light source and the light detector are located within a substantially completely lightproof compartment within said housing.

9. A plate reader according to claim 8, wherein the position indicating means comprise a second light source mounted on said carriage, but positioned externally of said lightproof compartment so as to be visible through a translucent window in the housing.

10. A plate reader according to claim 6, wherein said window is located in said housing in a plane located above and parallel with the microtitre plate, when in position therein, said window having markings thereon or associated therewith which, at any given position of the carriage, identify the particular well which, at that moment, is located between the light source and the detector.

11. A plate reader according to claim 1, wherein the absorbance indicating and/or recording means comprise a digital display indicating the absorbances of the samples under test and contained within the wells of the microtitre plate.

12. In a plate reader for microtitre plates of the type comprising an enclosed housing, means for receiving a microtitre plate within the housing in a position to be scanned by a reading head movable relative to the plate to bring said head into alignment with each of the wells of the microtitre plate in turn, illuminating means associated with said reading head for directing a beam of light through each of said wells in turn, and measuring means for measuring the amount of light transmitted by each of said wells in turn,

the combination of improvements comprising:

a substantially flat housing means having in its upper surface a window marked with an identifying grid enabling the identification of the individual wells of the microtitre plate when received within the housing;

a drawer slidably mounted in the housing means and slidable with respect thereto between an open position, thereby to permit the insertion therein and subsequent removal therefrom of a microtitre plate to be held therein in a fixed position by plate supporting means, and a closed position in which said plate is located totally within the housing means in a fixed position relative thereto in alignment with, and in a horizontal plane parallel to, but vertically spaced from and below said window;

a carriage means mounted in said housing means and manually movable thereon along two orthogonal axes at right angles one to the other by manually operable control means mounted externally of the housing, said carriage means comprising two parallel, vertically spaced apart arms lying in two parallel planes located above and below, respectively, the horizontal plane occupied by said microtitre plate when in position in the housing, said arms carrying at their distal ends, respectively, a light source and, vertically aligned therewith, a light detector means for detecting and measuring light transmitted through a respective one of the wells of the microtitre plate, when said light source and detector means are positioned in vertical alignment with said well, one immediately thereabove and the other immediately therebelow;

manually operable control means mounted externally of the housing and operably connected internally of the housing to said carriage and operable to move said carriage relative to the housing and said plate along said two orthogonal axes thereby to bring said light source and said detector means into vertical alignment with each of said wells in turn;

detent means for positively locating the carriage within said housing when said light source and detector means are in vertical alignment with each in turn of said wells; and

a position indicating means carried by the carriage and visible through said window to indicate the instantaneous position of the carriage inside the housing and to identify the particular well which, at that moment, is located between the light source and the detector and whose transmittance or adsorbance is to be measured.

13. A plate reader according to claim 12, wherein said light source is carried by the uppermost of said two vertically spaced apart arms and is visible through said window to provide said position indicating means.

14. In a plate reader according to claim 12, the further improvement comprising a lightproof baffle positioned in said housing and lying in a horizontal plane between said window and said microtitre plate when inserted in the housing, thereby to define within the housing a totally lightproof compartment for the microtitre plate.

15. A plate reader according to claim 14, wherein the position indicating means comprises a third arm on said carriage means parallel with but vertically spaced above the uppermost of said two arms carrying said light source and detector means respectively, said third arm lying in a horizontal plane between said window and said lightproof baffle and carrying at its distal end an indicator means vertically aligned with said light source and said detector means and visible through said window to indicate the position of the carriage and identify the particular well which at that moment lies between the light source and the detector means.

16. A plate reader according to claim 15, wherein said indicator means comprises a second light source carried on the distal end of said third arm and visible through said window, but shelded from the microtitre plate when in position in the housing by said lightproof baffle.

17. In a plate reader for microtitre plates of the type comprising an enclosed housing, means for receiving a microtitre plate within the housing in a position to be scanned by a reading head movable relative to the plate to bring said head into alignment with each of the wells of the microtitre plate in turn, illuminating means associated with said reading head for directing a beam of light through each of said wells in turn, and measuring means for measuring the amount of light transmitted by each of said wells in turn,

the combination of improvements comprising:

a substantially flat housing means;

a drawer slidably mounted in the housing means and slidable with respect therein between an open position, thereby to permit the insertion therein and subsequent removal therefrom of a microtitre plate to be held therein in a fixed position by plate supporting means, and a closed position in which said plate is located totally within the housing means in a fixed horizontal plane;

a carriage means mounted in said housing means and manually movable thereon along two orthogonal axes at right angles one to the other by manually operable control means mounted externally of the housing, said carriage means comprising two parallel, vertically spaced apart arms lying in two parallel planes located above and below, respectively, the horizontal plane occupied by said microtitre plate when in position in the housing, said arms carrying at their distal ends, respectively, a light source and, vertically aligned therewith, a light detector means for detecting and measuring light transmitted through a respective one of the wells of the microtitre plate, when said light source and detector means are positioned in vertical alignment with said well, one immediately thereabove and the other immediately therebelow;

manually operable control means mounted externally of the housing and operably connected internally of the housing to said carriage and operable to move said carriage relative to the housing and said plate along said two orthogonal axes thereby to bring said light source and said detector means into vertical alignment with each of said wells in turn;

detent means for positively locating the carriage within said housing when said light source and detector means are in vertical alignment with each in turn of said wells; and

a position indicating means to indicate the instantaneous position of the carriage inside the housing and to identify the particular well which, at that moment, is located between the light source and the detector and whose transmittance or adsorbance is to be measured.

18. A plate reader according to claim 17, wherein the substantially flat housing means has a window in its upper surface marked with an identifying grid enabling the identification of the individual wells of the microtitre plate which is received in said housing means in a fixed position immediately below and in alignment with said window, and wherein the position indicating means comprise an indicator means carried by the carriage and visible through said window to indicate the instantaneous position of the carriage inside the housing and to identify by reference to said grid the particular well which, at that moment, is located between the light source and the detector and whose transmittance or adsorbance is to be measured.