Enzyme sensor for determining a concentration of glutamate

• US 4,812,220 A
• Filed: 08/12/1987
• Issued: 03/14/1989
• Est. Priority Date: 08/14/1986
• Status: Expired due to Fees

• Current Assignee: Unitika Limited
• Original Assignee: Unitika Limited
• Inventors: Kawabe, Takeshi, Iida, Takeaki
• Primary Examiner(s): Tung, T.
• Application Number:
  US07/084,570
• Time in Patent Office: 580 Days
• Field of Search: 204/403, 204/416-420, 435/4, 435/817
• US Class Current: 204/403.1
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: G01N 27/4145 specially adapted for biomolecules, e.g. gate electrode with immobilised receptors
• CPC Class Codes: G01N 33/02 Food
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

Disclosed is an enzyme sensor for determining a concentration of glutamate comprising an immobilize enzyme acting specifically to a substrate and a transducer for converting the quantitative change of a substance or heat which is produced or consumed during an enzyme reaction to an electrical signal, wherein the enzyme is glutamine synthetase and the transducer is the pH glass electrode or ion-sensitive field-effect transistor (ISFET). The enzyme sensor can be miniaturized and can accurately determine a concentration of glutamate even when it is low.

Claims

1. An enzyme sensor for determining a concentration of glutamate comprising an immobilize enzyme acting specifically to a substrate and a transducer for converting the quantitative change of a substance or heat which is produced or consumed during an enzyme reaction to an electrical signal, wherein the enzyme is glutamine synthetase and the transducer is the pH glass electrode or ion-sensitive field-effect transistor.

2. The enzyme sensor according to claim 1 wherein glutamine synthetase is isolated from a microorganism having a most suitable growth temperature of 50°

to 85°

C.