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Hydrolyzable fluorescent substrates and analytical determinations using same

  • US 4,812,409 A
  • Filed: 01/31/1986
  • Issued: 03/14/1989
  • Est. Priority Date: 01/31/1986
  • Status: Expired due to Term
First Claim
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1. A hydrolyzable compound represented by the formula:

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    space="preserve" listing-type="equation">BLOCK--X--R.sup.f --Lwherein BLOCK is a hydrolyzable group selected from the group consisting of;

    --COR1 ;

    a monovalent moiety derived by removal of a hydroxy group from a carboxy group of an amino acid or peptide;

    or a monovalent moiety derived by removal of a hydroxy group from a mono- or polysaccharide,R1 is hydrogen, alkyl, alkenyl, aryl, cycloalkyl or a heterocyclic group,X is --O--, --S-- or --NR-- and is attached to BLOCK at the open valence on BLOCK wherein R is hydrogen, alkyl, cycloalkyl, phenyl or a heterocyclic group,Rf is a phenalenone or benzphenalenone fluorescent moiety selected from the group consisting essentially of ##STR6## provided that when released as --X--Rf --L, --X--Rf --L exhibits maximum fluorescent emission at least about 580 nm at a pH of 9 or less, and L is hydrogen or a specific binding ligand.

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