Optical sensor for selective detection of substances and/or for the detection of refractive index changes in gaseous, liquid, solid and porous samples

• US 4,815,843 A
• Filed: 01/27/1987
• Issued: 03/28/1989
• Est. Priority Date: 05/29/1985
• Status: Expired due to Term

• Current Assignee: Artificial Sensing Instruments ASI AG
• Original Assignee: Unaxis Holding AG (OC Oerlikon Corporation AG)
• Inventors: Tiefenthaler, Kurt, Lukosz, Walter
• Primary Examiner(s): Rosenberger, Richard A.
• Application Number:
  US07/019,557
• Time in Patent Office: 791 Days
• Field of Search: 356/128, 356/136, 350/96.15, 350/96.19
• US Class Current: 356/128
• CPC Class Codes: G01N 2021/437 with investigation of angle
• CPC Class Codes: G01N 21/431 Dip refractometers, e.g. using optical fibres
• CPC Class Codes: G01N 21/7743 the reagent-coated grating coupling light in or out of the waveguide
• CPC Class Codes: G02B 6/12007 forming wavelength selective elements, e.g. multiplexer, demultiplexer
• CPC Class Codes: G02B 6/124 Geodesic lenses or integrated gratings
• CPC Class Codes: G02B 6/34 utilising prism or grating G02B6/293 takes precedence

Abstract

The optical sensor for the detection of specific substances and refractive index changes in gaseous, liquid, solid and porous samples is composed of integrated optical elements. It consists of a waveguiding film (1) equipped with a diffraction grating (4) and applied onto a substrate (2). For the selective detection of a specific substance contained in the sample (3) a selectively chemisorbing additional layer (5) covers the film (1) at least in the grating region. The sample (3) is applied at least in the grating region either directly onto the waveguiding film (1) or onto the selectively chemisorbing additional layer (5). Chemisorption of the substance, which is contained in the sample (3) and is to be detected, to the selectively chemisorbing additional layer (5) results in a further adlayer (6) coating the waveguiding film (1). The principle of the sensor is as follows: Due to chemisorption of a further adlayer (6) and/or due to a refractive index change of the sample (3) a change in the effective refractive index N of a mode guided in the waveguiding film (1) is effected. For the detection of this change the set-up (1,2,3,4,5) described above is used as grating coupler or Bragg reflector.

Claims

1. An optical sensor for detecting chemical, biochemical or biological substances in a sample, comprising:

a waveguiding structure formed by a waveguiding film covering a substrate, wherein the waveguiding film has a refractive index at least 1% higher than the refractive index of the substrate;

a diffraction grating contained in the waveguiding structure; and

a chemo-responsive layer covering the waveguiding film in a region around the diffraction grating, wherein said chemo-responsive layer is capable of binding with the substances to be assayed and has a thickness of less than one wavelength.

2. An optical sensor according to claim 1, wherein the waveguiding structure outside the region around the diffraction grating is covered by a protective layer.

3. An optical sensor, according to claim 2, wherein the protective layer consists of SiO₂.

4. An optical sensor according to claim 3, wherein the protective layer tapers near the region around the diffraction grating.

5. An optical sensor according to claim 4, wherein the diffraction grating is a surface relief grating on the waveguiding structure.

6. An optical sensor according to claim 5, wherein the diffraction grating is a phase volume grating.

7. An optical sensor according to claim 6, wherein the waveguiding structure is a high index monomode structure.

8. An optical sensor according to claim 7, wherein the waveguiding film is covered by a binding layer.

9. An optical sensor according to claim 8, wherein the binding layer is a silane.

10. An optical sensor according to claim 9, wherein the chemo-responsive layer is a monomolecular layer.

11. An optical sensor according to claim 9, wherein the waveguiding structure is microporous in the region around the diffraction grating and the chemoresponsive molecules are brought into the micropores.

12. An optical sensor according to claim 11, wherein the substrate is microporous in the region around the diffraction grating and chemo-responsive molecules are brought into the micropores.

13. An optical sensor according to claim 11, wherein the chemo-responsive layer is composed of molecules of a specifically binding biological receptor that specifically blinds with biomolecules which are complementary to the receptor.

14. An optical sensor according to claim 13, wherein the chemo-responsive layer comprises antibodies for an antigen which is to be assayed.

15. An optical sensor according to claim 14, wherein a membrane of selective permeability is fixed on the protective layer.

16. An optical sensor according to claim 8, wherein the binding layer is a polymer.

17. An optical sensor according to claim 16, wherein the chemo-responsive layer is a monomolecular layer.

18. An optical sensor according to claim 16, wherein the waveguiding structure is microporous in the region around the diffraction and chemo-responsive molecules are brought into the micropores.

19. An optical sensor according to claim 18, wherein the substrate is microporous in the region around the diffraction grating and chemo-responsive molecules are brought into the micropores.

20. An optical sensor according to claim 18, wherein the chemo-responsive layer is composed of molecules of a specifically binding biological receptor that specifically binds biomolecules which are complementary to the receptor.

21. An optical sensor according to claim 20, wherein the chemo-responsive layer comprises antibodies for an antigen which is to be assayed.

22. An optical sensor according to claim 21, wherein a membrane of selective permeability is fixed on the protective layer.

23. A method for detecting chemical, biochemical or biological substances in a sample including the steps of:

measuring the effective index of an optical sensor comprising a waveguiding structure formed by a waveguiding film covering a substrate wherein the waveguiding film has a refractive index at least 1% higher than the refractive index of the substrate,

a diffraction grating in said waveguiding structure, and a chemo-responsive layer covering the waveguiding film in a region around the diffraction grating so that the chemo-responsive layer is capable of binding the substances to be assayed and has a thickness of less than one wavelength;

contacting said optical sensor with the sample; and

measuring the effective index change caused by the binding of the species to the chemo-responsive layer.

24. A method according to claim 23, wherein the maximum effective index change is measured in order to determine the concentration of the substances.

25. A method according to claim 24, in which the velocity of the effective index change is measured.

26. A method according to claim 25, wherein a laser beam is coupled into the waveguiding structure under a constant angle of incidence and the intensity change of the incoupled mode is measured.

27. A method according to claim 25, wherein a laser beam is coupled into the waveguiding structure under a constant angle of incidence and the intensity change of the non-incoupled diffraction order is measured.

28. A method according to claim 25, wherein a laser beam is coupled into the waveguiding structure under the angle of incidence leading to the highest intensity of the incoupled mode and measuring the effective index change by determining the change of the angle of incidence leading to highest intensity of the incoupled mode.

29. A method according to claim 25, wherein a laser beam is coupled into the waveguiding structure under a constant angle of incidence and the intensity change of the non-incoupled diffraction order is measured.

30. A method according to claim 25, wherein a laser beam is coupled into the waveguiding structure under a constant angle of incidence and the change of wavelength is measured.

31. A method according to claim 25, wherein a laser beam is coupled into the waveguiding structure and the change of the outcoupling angle is measured.