pH/P.sub.CO2 P.sub.O2 electrode

• US 4,816,131 A
• Filed: 09/29/1987
• Issued: 03/28/1989
• Est. Priority Date: 09/29/1987
• Status: Expired due to Fees

• Current Assignee: Board of Regents of The University of Washington (University of Washington)
• Original Assignee: Board of Regents of The University of Washington (University of Washington)
• Inventors: Bomsztyk, Karol
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US07/102,323
• Time in Patent Office: 546 Days
• Field of Search: 128/635, 204/412, 204/403
• US Class Current: 204/403.1
• CPC Class Codes: G01N 27/4162 investigating the composition of gases, by the influence exerted on ionic conductivity in a liquid conductometry in general G01N27/06; amperometric gas sensors G01N27/404
• CPC Class Codes: G01N 33/492 Determining multiple analytes

Abstract

A double and triple barrel electrode for the simultaneous measurement of pH and P_CO2 ; and pH, P_CO2 and P₀₂ employing a membrane in conjunction with a pH fluid and fluids responsive to CO₂ and O₂. The responsive fluids exhibit a pH change upon a change in the concentration of the particular analyte in the responsive fluid. The liquid membrane is selectively permeable to H⁺ ion, and permeable to CO₂ and O₂ gas.

Claims

1. An electrode device capable of simultaneously measuring pH and P_CO2 comprising:

(a) means defining a pH sensor, said pH sensor comprising a hydrogen ion and carbon dioxide gas permeable membrane in contact with a hydrogen ion responsive fluid, said fluid capable of being placed in contact with a means for measuring the voltage signal from the pH sensor;

(b) means defining a P_CO2 sensor, said P_CO2 sensor comprising a hydrogen ion and carbon dioxide permeable membrane having the same composition as the membrane in (a) in contact with a carbon dioxide responsive fluid, said fluid capable of being placed in contact with a means for measuring the voltage signal from the P_CO2 sensor; and

(c) wherein the means defining the pH sensor and the means defining the P_CO2 sensor are connected to each other.

3. The electrode device of claim 1, wherein said hydrogen ion responsive fluid is a solution consisting of sodium citrate/sodium chloride.

4. The electrode device of claim 1, wherein the means for measuring the voltage comprises a silver-silver chloride wire.

5. The electrode device of claim 1, wherein the carbon dioxide responsive fluid is a solution consisting of sodium bicarbonate/sodium chloride.

6. The electrode device of claim 1, wherein the means defining the pH sensor comprises a capillary tube.

7. The electrode device of claim 1, wherein the means defining the P_CO2 sensor comprises a capillary tube.

8. The device of claim 6 or 7, wherein the hydrogen ion and carbon dioxide gas permeable liquid membrane is a solution of o-nitrophenyl octyl ether containing about 10 g/100 g of tri-N-dodecylamine and about 0.7 g/100 g of sodium tetraphenylborate.

9. The electrode device of claim 8, wherein the hydrogen ion responsive fluid is a solution containing about 100 mM sodium citrate -100 mM sodium chloride.

10. The electrode device of claim 9, wherein the carbon dioxide responsive fluid is a solution containing about 20 mM sodium bicarbonate -100 mM sodium chloride.

11. The electrode device of claim 6, wherein the hydrogen ion and carbon dioxide gas permeable liquid membrane is used in an amount sufficient to occupy the lower portion of the capillary tube ranging in length from about 150 to about 250 microns.

12. The electrode device of claim 7, wherein the hydrogen ion and carbon dioxide gas permeable liquid membrane is used in an amount sufficient to occupy the lower portion of the capillary tube ranging in length from about 150 to about 250 microns.

13. The electrode device of claim 1, wherein the hydrogen ion responsive fluid is in contact with a means for measuring the voltage signal from the pH sensor.

14. The electrode device of claim 1, wherein the carbon dioxide ion responsive fluid is in contact with a means for measuring the voltage signal from the P_CO2 sensor.

15. The electrode device of claim 1, wherein the hydrogen ion and carbon dioxide gas permeable membrane comprises substrates selected from the group consisting of polyvinylchloride, silicones, polymethyl methacrylate, polyurethanes, and polystyrenes.

16. An electrode kit useful for simultaneously measuring pH and P_CO2 comprising sterile packaging and the electrode device of claim 1.

17. The electrode kit of claim 16, further comprising a pair of silver-silver halide wires.

18. The electrode kit of claim 17, further comprising instructions for use.

42. The electrode device of claim 1 or 19, wherein the hydrogen ion responsive fluid is a buffered solution whose pH is not affected by the presence of carbon dioxide gas or oxygen gas.

43. The electrode device of claim 1 or 19, wherein the pH of the carbon dioxide responsive fluid changes in response to a change in the P_CO2 of the carbon dioxide responsive fluid.

2. The electrode device of claim 2, wherein said hydrogen ion and carbon dioxide gas permeable membrane is a liquid membrane consisting of tri-N-dodecylamine and sodium tetraphenylborate in o-nitrophenyl octyl ether.

19. An electrode device for simultaneously measuring pH, P_CO2, and P_O2 comprising:

(a) means defining a pH sensor, said pH sensor comprising a hydrogen ion, carbon dioxide gas, and oxygen gas permeable membrane in contact with a hydrogen ion responsive fluid, said fluid capable of being placed in contact with a means for measuring the voltage signal from the pH sensor;

(b) means defining a P_CO2 sensor comprising a hydrogen ion, carbon dioxide gas and oxygen gas permeable membrane having the same composition as the membrane in (a) in contact with a carbon dioxide responsive fluid, said fluid capable of being placed in contact with a means for measuring the voltage signal from the P_CO2 sensor;

(c) means defining a P_O2 sensor, said P_O2 sensor comprising a hydrogen ion, carbon dioxide gas, and oxygen gas permeable membrane having the same composition as the membranes in (a) and (b) in contact with an oxygen responsive fluid, said fluid capable of being placed in contact with a means for measuring the voltage signal from the P_O2 sensor; and

(d) wherein the means defining the pH sensor, the means defining the P_CO2 sensor, and the means defining the P_O2 sensor are connected to each other.

20. The electrode device of claim 19, wherein said hydrogen ion, carbon dioxide gas and oxygen gas permeable membrane is a solution consisting of tri-N-dodecylamine and sodium tetraphenylborate in o-nitrophenyl octyl ether.

21. The electrode device of claim 20, wherein the means defining the pH sensor comprises a capillary tube.

22. The microelectrode device of claim 21, wherein the hydrogen ion, carbon dioxide gas, and oxygen gas permeable liquid membrane is used in an amount sufficient to occupy the lower portion of the capillary tube ranging in length from about 150 to about 250 microns.

23. The electrode device of claim 20, wherein the means defining the P_CO2 sensor comprises a capillary tube.

24. The microelectrode device of claim 23, wherein the hydrogen ion, carbon dioxide gas, and oxygen gas permeable liquid membrane is used in an amount sufficient to occupy the lower portion of the capillary tube ranging in length from about 150 to about 250 microns.

25. The electrode device of claim 20, wherein the means defining the P_O2 sensor comprises a capillary tube.

26. The microelectrode device of claim 25, wherein the hydrogen ion, carbon dioxide gas, and oxygen gas permeable liquid membrane is used in an amount sufficient to occupy the lower portion of the capillary tube ranging in length from about 150 to about 250 microns.

27. The electrode device of claim 20, wherein the hydrogen ion, carbon dioxide gas and oxygen gas permeable liquid membrane is a solution of o-nitrophenyl octyl ether containing about 10 grams/100 grams of tri-N-dodecylamine and about 0.7 grams/100 grams of sodium tetraphenylborate.

28. The electrode device of claim 27, wherein the hydrogen ion responsive fluid is a solution containing about 100 mM sodium citrate-100 mM sodium chloride.

29. The electrode device of claim 28, wherein the carbon dioxide responsive fluid is a solution containing about 20 mM sodium bicarbonate-100 mM sodium chloride.

30. The microelectrode device of claim 29, wherein the oxygen responsive fluid is a solution of about 1.0×

10^-3 M iron sulfate salt dissolved in water.

31. The electrode device of claim 19, wherein said hydrogen ion responsive fluid is a solution consisting of sodium citrate/sodium chloride.

32. The electrode device of claim 19, wherein the means for measuring the voltage comprises a silver-silver chloride wire.

33. The electrode device of claim 19, wherein the carbon dioxide responsive fluid is a solution of sodium bicarbonate/sodium chloride.

34. The electrode device of claim 19, wherein the oxygen responsive fluid is a solution containing at least one component selected from the group consisting of ferrous ion, glucose/glucose oxidase, reduced nicotinamide adenin dinucleotide, and hemoglobin.

35. The electrode device of claim 19, wherein the hydrogen ion responsive fluid is in contact with a means for measuring the voltage signal from the pH sensor.

36. The electrode device of claim 19, wherein the carbon dioxide ion responsive fluid is in contact with a means for measuring the voltage signal from the P_CO2 sensor.

37. The electrode device of claim 19, wherein the oxygen ion responsive fluid is in contact with a means for measuring the voltage signal from the P_O2 sensor.

38. The electrode device of claim 19, wherein the hydrogen ion, carbon dioxide gas and oxygen gas permeable membrane comprises substrates selected from the group consisting of polyvinylchloride, silicones, polymethyl methacrylate, polyurethanes, and polystyrenes.

39. An electrode kit useful for simultaneously measuring pH, P_CO2, and P_O2 comprising sterile packaging and the microelectrode device of claim 19.

40. The electrode kit of claim 39, further comprising a pair of silver-silver halide wires.

41. The electrode kit of claim 39, further comprising instructions for use.

44. The electrode device of claim 19, wherein the pH of the oxygen sensitive responsive fluid changes in response to a change in the P_O2 of the oxygen responsive fluid.