Device for separating plasma or serum from whole blood and analyzing the same

• US 4,816,224 A
• Filed: 07/31/1987
• Issued: 03/28/1989
• Est. Priority Date: 08/05/1980
• Status: Expired due to Term

• Current Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Original Assignee: Boehringer Mannheim GmbH (Roche Holding AG)
• Inventors: Braun, Hans-Peter, Werner, Wolfgang, Vogel, Peter, Berger, Dieter
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): Hill, Jr., Robert J.
• Application Number:
  US07/081,624
• Time in Patent Office: 606 Days
• Field of Search: 422/55-58, 422/101, 436/170, 436/177, 436/178, 73/64.1, 210/500.1, 210/503, 210/505, 210/508, 210/509, 210/767, 210/782, 210/927
• US Class Current: 422/422
• CPC Class Codes: B01D 39/2017 the material being filamentary or fibrous
• CPC Class Codes: G01N 33/48 Biological material, e.g. blood, urine G01N33/02, G01N33/26, G01N33/44, G01N33/46 take precedence; Haemocytometers counting blood corpuscules distributed over a surface by scanning the surface G06M11/02
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A device for separating plasma or serum from whole blood and analyzing the same, wherein the device includes a layer of glass fibers with an average diameter of from 0.2 to 5 microns and having a density of from 0.1 to 0.5 g./cm³ and an adsorption volume at least twice that of the plasma or serum to be separated and, preferably, more than 3.3 times the volume.

Claims

1. A unitary multilayer device for separating plasma or serum from whole blood and analyzing such plasma or serum, comprising:

(a) a fibrous layer capable of separating blood cells from serum or plasma in a whole blood sample, wherein said fibrous layer contains glass fibers and has a density of from 0.1 to 0.5 g/cm³ and wherein said glass fibres have an average diameter of from 0.2 to 5 microns; and

(b) a reaction layer which is or may be brought into fluid communication with said fibrous layer while leaving at least a portion of said fibrous layer free from direct attachment to said reaction layer, said reaction layer contaning means for determining a component of plasma or serum.

2. The device of claim 1, further comprising an inert substrate to which said fibrous layer and said reaction layer are attached directly or indirectly.

3. The device of claim 1, wherein said glass fibers have an average diameter of from 0.5 to 2.5 microns.

4. The device of claim 1, wherein said glass fibers have an average diameter of from 0.5 to 1.5 microns.

5. The device of claim 1, wherein said fibrous layer further comprises synthetic resin fibers.

6. The device of claim 1, wherein said reactin layer contains a plurality of layers.

7. The device of claim 1, further comprising an inert carrier between said fibrous layer and said reaction layer which carrier permits passage of plasma or serum into said reaction layer.

8. The device of claim 1, further comprising an inert carrier layer positioned below said reaction layer wherein said reaction layer and said inert carrier are adapted to produce a detection reaction with a component material of plasma or serum which is analyzable or detectable through said inert carrier.

9. Device of claim 1, further comprising a removable layer which supports said glass fiber containing fibrous layer.

10. The device of claim 9, further comprising means for fixing said removable layer to a substrate.