Continuous process for ethanol production by bacterial fermentation

• US 4,816,399 A
• Filed: 04/12/1985
• Issued: 03/28/1989
• Est. Priority Date: 04/12/1985
• Status: Expired due to Fees

• Current Assignee: George Weston Limited
• Original Assignee: George Weston Limited
• Inventors: Lawford, Hugh G.
• Primary Examiner(s): Warren, Charles F.
• Assistant Examiner(s): Marx, Irene
• Application Number:
  US06/722,492
• Time in Patent Office: 1,446 Days
• Field of Search: 435/161, 435/244, 435/253, 435/822, 435/813
• US Class Current: 435/161
• CPC Class Codes: C12M 41/32 of substances in solution
• CPC Class Codes: C12P 7/065 with microorganisms other than yeasts
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel
• CPC Class Codes: Y10S 435/813 Continuous fermentation
• CPC Class Codes: Y10S 435/822 using bacteria or actinomycetales

Abstract

A continuous process for the production of ethanol by fermentation with strains of Zymomonas is provided. Metabolic processes are limited by the nutrients nitrogen, potassium and phosphorus. When growth is limited by one of these nutrients, the biomass expresses its maximum value for both q_s and q_p at any given value of D and S_r. The process is conducted at a lower biomass concentration and a higher specific rate of ethanol formation than a similar process conducted with a nutrient medium that is not limited in nitrogen, potassium or phosphorus. A method of improving performance of Zymomonas in continuous ethanol fermentation at increased temperatures is also provided.

Claims

1. A continuous process for the production of ethanol, which comprises cultivating in submerged culture under anaerobic conditions an organism of the Zymomonas genus in an aqueous nutrient medium containing potassium and assimilable carbon, nitrogen and phosphorus until a recoverable quantity of ethanol is produced;

wherein said nutrient is limited in one or more of the nutrients selected from the group consisting of nitrogen, potassium and phosphorus such that the rate of growth of said organism is limited by the availability of the limited nutrient;

and wherein said biomass expresses its maximal value for both q_s and q_p under the condition of nutrient limitation;

said process being conducted as a nutrient-limited fermentation and at a lower biomass concentration and a higher specific rate of ethanol formation than a similar process conducted with a nutrient medium that contains said nutrients in excess, and wherein each of said processes is carried out at the same fermentation temperature, dilution rate and concentration of assimilable carbon.

2. Process according to claim 1 wherein fermentation is conducted at a temperature of about 27°

C. to about 37°

C.

3. Process according to claim 1 wherein fermentation is conducted in a fermentation medium having a pH of about 4.5 to about 6.5.

4. Process according to claim 1 wherein fermentation is conducted at a temperature of about 30°

C. and a pH of about 5.5.

5. Process according to claim 1 wherein the nutrient medium contains an approximate amount of limiting nutrient according to the following relationships ##EQU3##

6. Process according to claim 1 wherein said organism is a Z. mobilis strain selected from the group consisting of ATCC 10988, ATCC 29191, ATCC 31821 and ATCC 31822.

7. Process according to claim 1 wherein said organism is Z. mobilis strain ATCC 29191.

8. Process according to claim 1 wherein said substrate is comprised of glucose.

9. Process according to claim 8 wherein substrate concentration in the fermentation medium is about 100 g/L to about 180 g/L.

10. Process according to claim 9 wherein concentration of substrate in effluent from the process is less than about 5% S_r.

11. Process according to claim 9 wherein effluent from the process is substantially free of substrate.

12. Process according to claim 1 wherein concentration of ethanol in the fermentation medium is about 50 g/L to about 60 g/L.

13. Process according to claim 1 wherein fermentation is carried out in a fermenter with cell recycle.

14. Process according to claim 1 wherein said nitrogen is derived from ammonium chloride, ammonium sulphate or mixtures thereof.

15. Process according to claim 1 wherein said phosphorus is derived from KH₂ PO₄.

16. Process according to claim 1 wherein said potassium is derived from KCl.

17. Process according to claim 4 wherein said organism is Z. mobilis strain ATCC 29191.

18. Process according to claim 17 wherein said substrate is comprised of glucose.

19. Process according to claim 18 wherein substrate concentration in the fermentation medium is about 100 g/L to about 180 g/L.

20. Process according to claim 19 wherein effluent from the process is substantially free of substrate.

21. Process according to claim 20 wherein concentration of ethanol in the fermentation medium is about 50 g/L to about 60 g/L.

22. Process according to claim 21 wherein fermentation is carried out in a fermenter with cell recycle.