Method and apparatus for performing automated, multi-sequential immunoassays

• US 4,816,418 A
• Filed: 07/22/1985
• Issued: 03/28/1989
• Est. Priority Date: 07/22/1985
• Status: Expired due to Fees

• Current Assignee: Sequoia-Turner Corp. (Abbott Laboratories Incorporated)
• Original Assignee: Sequoia-Turner Corp. (Abbott Laboratories Incorporated)
• Inventors: Konicek, Michael G., Mack, Daniel R.
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US06/757,676
• Time in Patent Office: 1,345 Days
• Field of Search: 436/800, 436/808, 436/518, 436/548, 422/65, 422/69
• US Class Current: 436/518
• CPC Class Codes: G01N 2035/00772 mechanical or optical code other than bar code
• CPC Class Codes: G01N 35/00732 Identification of carriers, materials or components in automatic analysers
• CPC Class Codes: G01N 35/026 having blocks or racks of reaction cells or cuvettes
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/808 Automated or kit

Abstract

Methods and apparatus useful in automated performance of immunoassays, in some preferred embodiments, relying upon the sandwich immunoassay technique, are described. Each rack of reaction tubes is uniquely identified by an optical identifier disc which is read by the automatic controller. The assays are performed at two stations which configuration thereby permits arrays to be run on a second rack of reaction tubes while the first rack is in process. The cooperation of the identifier disc and the automatic controller minimizes operator error and helps improve the reliability of the data obtained.

Claims

1. A method for automatically performing immunoassay techniques on a rack of reaction tubes, the method comprising the steps of:

identifying uniquely a rack of reaction tubes;

communicating to an automatic controller the immunoassay technique to be performed on said rack of reaction tubes;

automatically communicating to the controller the unique identity of said rack;

loading the rack to a first station;

I. at the first station, according to the particular immunoassay technique selected,

(a) automatically adding sample serum to each of said reaction tubes to permit the sample serum to contact a substance which will immunochemically bond to a specific immunogenic component of said sample serum;

(b) automatically adding a first reagent to said reaction tubes which reagent also immunochemically bonds to said specific immunogenic component of the sample serum to form a first complex;

(c) removing said rack of reaction tubes from the first station;

incubating said reaction tubes for a first period of time;

loading the rack to a second station;

automatically verifying by said controller the unique identity of said rack; and

II. at the second station, according to the particular immunoassay technique originally selected,

(a) automatically washing away unreacted components of the sample serum and reagents in each reaction tube to leave said first complex;

(b) automatically adding a second reagent to said washed reaction tubes which second reagent results in the formation of an optically detectable substance;

(c) incubating the rack of reaction tubes for a second period of time;

(d) automatically adding a diluent and/or a quenching reagent to said reaction tubes; and

(e) automatically transfering the contents of each reaction tube to an optics module; and

(f) obtaining and presenting an optical characterization of the contents of each of said reaction tubes.

2. The method of claim 1 wherein the substance of step I (a) binds to a solid support.

3. The method of claim 1 wherein the substance of step I, (a) is a monoclonal antibody.

4. The method of claim 1 wherein the specific immunogenic component of step I, (a) is a specific antigen.

5. The method of claim 1 wherein the second reagent of step II, (b) is a chromophore-forming substrate which is activated in the presence of the first reagent.

6. The method of claim 1 wherein an additional step of communicating with the controller to determine when the first incubation period will end is performed after beginning the first incubation period for said reaction tubes.

7. The method of claim 1 wherein said controller comprised, in part, interactive components and memory storage.

8. The method of claim 1 wherein said rack identifying and verifying steps comprise using an optical marker on the rack of reaction tubes and automatically, optically sensing the marker.

9. The method of claim 1 wherein the step of obtaining and presenting an optical characterization of the contents of each of said reaction tubes is performed by an optics module.

10. An apparatus for automatically performing immunoassay techniques on a rack of reaction tubes, which comprises:

(a) means for identifying uniquely a rack of reaction tubes;

(b) an automatic controller;

(c) means for automatically communicating to the controller the unique identity of said rack;

(d) a first station;

(e) means for automatically adding sample serum to each of said reaction tubes;

(f) means for automatically adding a first reagent to said reaction tubes;

(g) means for automatically verifying by said controller the unique identity of said rack;

(h) a second station;

(i) means for automatically washing away unreacted components of the sample serum and reagents in each reaction tube to leave said first complex;

(j) means for automatically adding a second reagent to said washed reaction tubes;

(k) automatically adding a diluent and/or quench reagent to said reaction tubes;

(l) an optics module;

(m) means for automatically transfering the contents of each reaction tube to an optics module; and

(n) obtaining and presenting an optical characterization of the contents of each of said reaction tubes.

11. The apparatus of claim 10 wherein said controller comprises, in part, interactive components and memory storage.

12. The apparatus of claim 10 wherein said rack identifying means comprises an optical marker located on one side of the rack of reaction tubes.

13. The apparatus of claim 10 wherein said automatic rack verifying means comprises an optical sensor which is capable of identifying locations of an optical marker on the rack.

14. The apparatus of claim 10 wherein said optics module comprises a spectrophotometer.

15. The apparatus of claim 10 wherein said optics module comprises a spectrofluorometer.