Luminometer apparatus

• US 4,818,883 A
• Filed: 12/16/1987
• Issued: 04/04/1989
• Est. Priority Date: 12/17/1986
• Status: Expired due to Fees

• Current Assignee: Firstpool Limited
• Original Assignee: Biolite LLC
• Inventors: Applebaum, Martin, Anderson, Joseph C.
• Primary Examiner(s): FIELDS, CAROLYN
• Application Number:
  US07/133,569
• Time in Patent Office: 475 Days
• Field of Search: 307/351, 328/115, 250/458.1, 250/459.1, 250/361 C, 436/172, 422/52, 356/317, 356/318, 356/417
• US Class Current: 250/361.00C
• CPC Class Codes: G01J 2001/446 Photodiode
• CPC Class Codes: G01N 2021/0162 using microprocessors for control of a sequence of operations, e.g. test, powering, switching, processing
• CPC Class Codes: G01N 2021/0168 for the measurement cycle
• CPC Class Codes: G01N 2021/0175 for selecting operating means
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence

Abstract

A luminometer apparatus has a light-tight box (12) into which a cuvette (2) containing a sample and a reagent are located for producing a phosphorescent reaction. The phosphorescent reaction is detected by a photodetector (PD) and a shutter (3) is interposed between the cuvette and the shutter. Output signals from the photodetector are applied to a circuit arrangement (FIG. 4) which detects the phosphorescent reaction at predetermined intervals and by subtracting the successive values of light from one another so a measured peak value of light intensity is determined. The shutter is closed and the dark current signal of the photodetector is determined and subtracted from the measured peak value. A true peak intensity of the emitted light is thus derived and the concentration of the material being assayed is calculated by raising the peak intensity to the power n where n has a value between 0 and 9 predeterminedly derived for the sample.

Claims

1. A luminometer apparatus including circuit means comprising a photodetector arranged to detect light emission from a phosphorescent reaction exhibiting a peak light value, means for predeterminedly shielding said photodetector from light, means arranged to sample the light emission from the phosphorescent reaction detected by the photodetector at predetermined time intervals when the shielding means is not shielding the photodetector, subtracting means connected to said sample means for subtracting the sampled light emission of consecutive samples and for determining the peak light value, means for moving the shielding means to shield said photodetector so as to detect the dark current of the photodetector after the peak light value has been determined and said subtracting means operable for subtracting the dark current from said peak light value to determine a true intensity of the peak light value.

2. An apparatus as claimed in claim 1, wherein there is provided (a) a microprocessor for determining the concentration of an analyte which is reacted with a reagent, said microprocessor calculating Iⁿ, where I is the true peak light value and n is a variable between 0 and 9, and (b) input means for inputting n to the microprocessor.

3. An apparatus as claimed in claim 2, wherein a display is provided arranged to display the output of the microprocessor which is one of the detected light emission from the photodetector, the photodetector dark current, the concentration of the sample, the value of the concentration with respect to a predetermined value.

4. An apparatus as claimed in claim 3, wherein the shutter is an electromechanical shutter comprising a motor driven rotatable member with an aperture therein.

5. An apparatus as claimed in claim 1, wherein a phosphorescent reaction container is provided and said shielding means is a shutter located between the photodetector and the phosphorescent reaction container wherein when the shutter is open the photodetector detects light from the reaction and when the shutter is closed the photodetector dark current is determinable.

8. A method as claimed in claim 1, wherein said predetermined time intervals are 5 seconds.

9. A method as claimed in claim 8, wherein the signal produced when no light falls on the photodetector is the photodetector dark current.

10. A method as claimed in claim 9, wherein a phosphorescent reaction is arranged to determine the concentration of an analyte by calculating Iⁿ where I is the peak light value and n is a variable value between 0 and 9 which is inputted to a microrpocessor wherein the concentration of the analyte is evaluated by the micrprocessor after the dark current signal has been subtracted from the measured peak light signal.

6. A luminometer apparatus including circuit means comprising a photodetector arranged to detect light emission from a phosphorescent reaction exhibiting a peak light value, means for predeterminedly shielding said photodetector from light, means arranged to sample the light emission from the phosphorescent reaction detected by the photodetector at predetermined time intervals when the shielding means is not shielding the photodetector, subtracting means connected to said sample means for subtracting the sampled light emission of consecutive samples and for determining the peak light value, means for moving the shielding means to shield the photodetector so as to detect the dark current of the photodetector after the peak light value has been reached, said subtracting means operable for subtracting the dark current from said peak light value, a microprocessor for determining the concentration of an analyte which is reacted with a reagent, said microprocessor calculating Iⁿ, where I is the true peak light value and n is a variable between 0 and 9, and input means for inputting n to the microprocessor.

7. A method of determining the peak light value of a phosphorescent reaction by the steps of using a photodetector to detect the light signal output from the phosphorescent reaction at predetermined time intervals, subtracting successive light output signals from one another, determining when the difference between successive light output readings falls below a predetermined minimum value to thereby determine the peak signal, determining the signal produced by the photodetector when no light falls thereon, and subtracting said produced signal from the peak signal to derive the true intensity of the peak light value.

11. A method of determining the peak light value of a phosphorescent reaction by the steps of using a photodetector to detect the light signal output from the phosphorescent reaction at predetermined time intervals, subtracting successive light output signals from one another, determining when the difference between successive light output readings falls below a predetermined minimum value to thereby determine the peak signal, determining the signal produced by the photodetector when no light falls thereon, subtracting said produced signal from the peak signal to derive the true intensity of the peak light value, and determining the concentration of an analyte which is reacted with a reagent by calculating Iⁿ where I is the true peak light value and n is an inputted value between 0 and 9.