Transdermal detection system

• US 4,821,733 A
• Filed: 08/18/1987
• Issued: 04/18/1989
• Est. Priority Date: 08/18/1987
• Status: Expired due to Fees

• Current Assignee: Dermal Systems International, Inc.
• Original Assignee: Dermal Systems International
• Inventors: Peck, Carl C.
• Primary Examiner(s): Wayner, William E.
• Application Number:
  US07/087,699
• Time in Patent Office: 609 Days
• Field of Search: 128/632, 128/760, 128/636, 128/635
• US Class Current: 600/361
• CPC Class Codes: A61B 5/14521 using means for promoting sweat production, e.g. heating the skin measuring sweat secretion A61B5/4266
• CPC Class Codes: A61B 5/14532 for measuring glucose, e.g. by tissue impedance measurement
• CPC Class Codes: C12Q 1/28 involving peroxidase
• CPC Class Codes: C12Q 1/54 involving glucose or galactose
• CPC Class Codes: C12Q 2326/12 3,3',5,5'-Tetramethylbenzidine, i.e. TMB
• CPC Class Codes: G01N 33/50 Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing measuring or testing processes involving enzymes or microorganisms, compositions or test papers therefor; processes for forming such compositions, condition responsive control in microbiological or enzymological processes C12Q
• CPC Class Codes: G01N 33/5302 Apparatus specially adapted for immunological test procedures

Abstract

A transdermal detection system for the detection of a target substance which migrates to the surface of the skin of a subject by diffusion comprises detector means and attachment means. The detector means includes at least one detector chemical contained in solution and capable of chemically reacting with the target substance as the target substance migrates to the skin surface of the subject to produce a detectable signal, and a barrier means for substantially preventing migration of the detector chemical into the skin surface of the subject. The attachment means maintains the detector means adjacent the surface of the skin of the subject.

Claims

1. An apparatus for the detection of a target substance which migrates to the surface of the skin of a subject by diffusion, comprising

detector means including (a) at least one detector chemical contained in solution and capable of chemically reacting with the target substance as the target substance migrates to the surface of the skin of the subject to produce a detectable signal and (b) a barrier means for substantially preventing said detector chemical from migrating into the skin of the subject;

and

attachment means for maintaining the detector means adjacent the surface of the skin of the subject.

2. An apparatus as defined by claim 1, wherein the detector means is capable of chemically reacting with the target substance as the target substance migrates to the surface of the skin of the subject to produce an electrically detectable signal.

3. An apparatus as defined by claim 2, wherein the detector means is capable of chemically reacting with the target substance as the target substance migrates to the surface of the skin of the subject to produce an electrically detectable change in an ion concentration in the detector means.

4. An apparatus as defined by claim 1, wherein the detector means further comprises a carrier substantially saturated with the detector chemical contained in solution and capable of chemically reacting with the target substance as the target substance migrates to the surface of the skin of the subject.

5. An apparatus as defined by claim 4, wherein the carrier comprises a porous support member.

6. An apparatus as defined by claim 5, wherein the carrier comprises a microporous membrane containing covalent functional groups capable of covalently binding with the detector chemical contained in solution.

7. An apparatus as defined by claim 1, wherein the barrier means comprises a permeable or a semipermeable membrane.

8. An apparatus as defined by claim 1, wherein the barrier means comprises a layer of gel.

9. An apparatus as defined by claim 1, wherein the barrier means comprises a microporous membrane containing covalent functional groups capable of covalently binding with the detector chemical contained in solution.

10. An apparatus as defined by claim 1, wherein the detector chemical is selected from chemical reagents, antibodies, enzymes and biological receptors and is contained in solution in a solvent.

11. An apparatus as defined by claim 1, wherein the detector means includes a mixture of detector chemicals contained in solution and capable of chemically reacting with the target substance as the target substance migrates to the surface of the skin of the subject to produce a detectable signal.

12. An apparatus as defined by claim 1, wherein the attachment means includes an adhesive area for adhesively maintaining the detector means adjacent the surface of the skin of the subject.

13. An apparatus as defined by claim 1, further comprising an impermeable cover means covering the surfaces of the detector means which are not positionable adjacent the surface of the skin of the subject.

14. An apparatus as defined by claim 1, further including a removable impermeable cover means covering the surface of the detector means which is positionable adjacent the surface of the skin of the subject, which removable cover means is adapted to be removed from the apparatus prior to use for the detection of a target substance migrating to the surface of the skin of the subject.

15. An apparatus as defined by claim 1, further including a signalling means capable of converting the detectable signal produced by the detector means into a form which is observable by the human eye.

16. An apparatus as defined by claim 15, wherein the signalling means includes microprocessing means.

17. An apparatus for the detection of a target substance which migrates to the surface of the skin of a subject by diffusion, comprising

detector means including (a) at least one detector chemical contained in solution and capable of chemically reacting with the target substance as the target substance migrates to the surface of the skin of the subject to produce an optically detectable signal and (b) a barrier means for substantially preventing said detector chemical from migrating into the skin of the subject;

and

attachment means for maintaining the detector means adjacent the surface of the skin of the subject.

18. An apparatus as defined by claim 17, wherein the detector means is capable of chemically reacting with the target substance as the target substance migrates to the surface of the skin of the subject to produce a visually detectable color change in the detector means.

19. An apparatus for the detection of a target substance which migrates to the surface of the skin of a subject by diffusion, comprising

detector means including (a) at least one detector chemical contained in solution and capable of chemically reacting with the target substance as the target substance migrates to the surface of the skin of the subject to produce a detectable signal, said detector chemical being selected from chemical reagents, antibodies, enzymes and biological receptors contained in solution in a solvent and including an oxidase in solution in a solvent, and (b) a barrier means for substantially preventing said detector chemical from migrating into the skin of the subject;

and

attachment means for maintaining the detector means adjacent the surface of the skin of the subject.

20. An apparatus as defined by claim 19, wherein the oxidase is selected from alcohol oxidases and glucose oxidases.

21. An apparatus for the detection of a target substance which migrates to the surface of the skin of a subject by diffusion, comprising

detector means including (a) at least one detector chemical including a mixture of an oxidase and a peroxidase contained in solution in an aqueous solvent and capable of chemically reacting with the target substance as the target substance migrates to the surface of the skin of the subject to produce a detectable signal and (b) a barrier means for substantially preventing said detector chemical from migrating into the skin of the subject;

and

attachment means for maintaining the detector means adjacent the surface of the skin of the subject.

22. An apparatus as defined by claim 21, wherein the oxidase is selected from alcohol oxidases and glucose oxidases.

23. An apparatus as defined by claim 21, wherein the peroxidase comprises horseradish peroxidase.

24. An apparatus for the detection of ethanol which migrates to the surface of the skin of a subject by diffusion, comprising

detector means including (a) detector chemicals comprising an alcohol oxidase, horseradish peroxidase and tetramethylbenzidine contained in solution and capable of chemically reacting with the ethanol as the ethanol migrates to the surface of the skin of the subject to produce a detectable color change and (b) a barrier means for substantially preventing migration of said detector chemicals into the skin of the subject;

and

attachment means for maintaining the detector means adjacent the surface of the skin of the subject.

25. An apparatus for the detection of glucose which migrates to the surface of the skin of a subject by diffusion, comprising

detector means including (a) detector chemicals comprising glucose oxidase, horseradish peroxidase and tetramethylbenzidine contained in solution and capable of chemically reacting with the glucose as the glucose migrates to the surface of the skin of the subject to produce a detectable color change and (b) a barrier means for substantially preventing migration of said detector chemicals into the skin of the subject;

and

attachment means for maintaining the detector means adjacent the surface of the skin of the subject.

26. A method for the detection of a target substance which migrates to the surface of the skin of a subject by diffusion, comprising maintaining a detector means adjacent the surface of the skin of the subject, the detector means including (a) at least one detector chemical contained in solution and capable of chemically reacting with the target substance as the target substance migrates to the surface of the skin of the subject to produce a detectable signal, and (b) a barrier means for substantially preventing migration of said detector chemical into the skin of the subject.