Carrier device for immunological assay

• US 4,822,565 A
• Filed: 03/11/1985
• Issued: 04/18/1989
• Est. Priority Date: 05/04/1984
• Status: Expired due to Fees

• Current Assignee: Dora Kohler
• Original Assignee: Dora Kohler
• Inventors: Kohler, Dora
• Primary Examiner(s): Rosen, Sam
• Assistant Examiner(s): Saunders, David A.
• Application Number:
  US06/710,123
• Time in Patent Office: 1,499 Days
• Field of Search: 436/530, 436/807, 436/809, 436/810, 422/56, 422/57, 422/58, 422/104, 427/2
• US Class Current: 422/425
• CPC Class Codes: G01N 33/544 the carrier being organic
• CPC Class Codes: G01N 33/548 Carbohydrates, e.g. dextran
• CPC Class Codes: G01N 35/1065 Multiple transfer devices
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays
• CPC Class Codes: Y10S 436/81 Tube, bottle, or dipstick

Abstract

A carrier coated with antibodies or antigens is proposed, which performs a serological reaction with human, animal or plant material to be investigated and which is received in a multi-well plate. The carrier is made from nitrocellulose or a material having the adsorption characteristics of nitrocellulose and its shape is adapted to the multi-well plate used for performing serological reactions and is introduced into the latter. A plastic reinforcing structure provides support for the coated carrier. The coated carrier is not completely bound to the plastic reinforcing structure, so that both sides of the carrier will be contacted by material in the multi-well plate.

Claims

1. A device adapted for use with a multi-well plate with individual wells for performing immunological assays, said device comprising

(a) a holding member,

(b) a flexible carrier and

(c) a plastic reinforcing structure;

said flexible carrier and said plastic reinforcing structure being attached to said holding member;

said flexible carrier comprising a plurality of strips formed of nitrocellulose or nitrocellulose-like material having the absorption characteristics of nitrocellulose;

said strips being arranged in comb-like, spaced fashion and being coated with antigens or antibodies and being capable of being immersed into individual wells of a multi-well plate;

said plastic reinforcing structure comprising a plurality of strips arranged in a comb-like, spaced fashion and being capable of being immersed into individual wells of a multi-well plate;

wherein each one of the plurality of flexible carrier strips is in a juxtaposed position relative to a plastic reinforcing strip but not completely bound thereto, so that both sides of the carrier strip will be contacted by the material in the wells and the plastic reinforcing strips will serve as a mechanical support.

2. A device according to claim 1 wherein said flexible carrier is nitrocellulose.