Cells labeled with multiple Fluorophores bound to a nucleic acid carrier
First Claim
1. A labeled cell consisting essentially of a cell, an antibody specific to and bound to such cell, a nucleic acid fragment covalently coupled to said antibody, and a plurality of fluorophores on said nucleic acid fragment, wherein said fluorophores are coupled to said nucleic acid fragment via a nucleic acid specific intercalator carrying a primary amine residue.
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Accused Products
Abstract
In passing labeled cells through a cell sorter, the improvement which comprises employing a labeled cell comprising a cell, an antibody specific to and bound to such cell, a nucleic acid fragment joined to said antibody, and a plurality of labels on said nucleic acid fragment. Because of the presence of multiple labels, the sensitivity of the separation of labeled cells is increased.
126 Citations
5 Claims
- 1. A labeled cell consisting essentially of a cell, an antibody specific to and bound to such cell, a nucleic acid fragment covalently coupled to said antibody, and a plurality of fluorophores on said nucleic acid fragment, wherein said fluorophores are coupled to said nucleic acid fragment via a nucleic acid specific intercalator carrying a primary amine residue.
Specification