Method and apparatus for measuring immunological reaction with the aid of fluctuation in intensity of scattered light

• US 4,826,319 A
• Filed: 05/23/1988
• Issued: 05/02/1989
• Est. Priority Date: 09/07/1984
• Status: Expired due to Fees

• Current Assignee: Olympus Optical Company Ltd. (Olympus Corporation)
• Original Assignee: Olympus Optical Company Ltd. (Olympus Corporation)
• Inventors: Ohno, Masahiro, Karaki, Kouichi, Uchino, Fumio, Tateoka, Hitoshi, Ando, Outaro, Namba, Akihiro, Nagasaki, Tatsuo
• Primary Examiner(s): Willis, Davis L.
• Assistant Examiner(s): Koren, Matthew W.
• Application Number:
  US07/197,336
• Time in Patent Office: 344 Days
• Field of Search: 356/336, 356/338, 356/339, 356/341, 356/434, 356/425, 356/246, 250/564, 250/565, 250/574, 422/64, 422/65, 422/73, 436/164
• US Class Current: 356/339
• CPC Class Codes: G01J 3/4412 Scattering spectrometry particle sizing by light scattering G01N15/0205; optical velocimetry of particles G01P5/20, G01P5/26
• CPC Class Codes: G01N 15/0205 by optical means
• CPC Class Codes: G01N 2015/0216 from fluctuations of diffraction pattern
• CPC Class Codes: G01N 2021/0325 Cells for testing reactions, e.g. containing reagents
• CPC Class Codes: G01N 2021/5969 Scanning of a tube, a cuvette, a volume of sample
• CPC Class Codes: G01N 2021/825 Agglutination
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/51 inside a container, e.g. in an ampoule G01N21/53 takes precedence
• CPC Class Codes: G01N 21/82 producing a precipitate or turbidity
• CPC Class Codes: G01N 2201/08 Optical fibres; light guides
• CPC Class Codes: G01N 33/53 Immunoassay; Biospecific binding assay; Materials therefor
• CPC Class Codes: G01N 35/00029 provided with flat sample substrates, e.g. slides G01N35/028 takes precedence

Abstract

A coherent laser light flux is projected into a cell made of transparent quartz and light scattered from particles suspended in an antigen-antibody reaction liquid contained in the cell is detected by a photomultiplier by means of a collimator. An output electrical signal from the photomultiplier is sampled at different time instances and samplings are supplied to a fast Fourier transformer to derive a plurality of power spectrum desnities of fluctuation in intensity of the scattered light. A plurality of power spectrum densities are averaged to generate a mean power spectrum density. An amount of antigen contained in the reaction liquid is measured in accordance with the mean power spectrum density.

Claims

1. An automatic analyzer for measuring antigen-antibody reaction comprising

means for feeding a plurality of cells along a given reaction line;

sample delivery means for delivering given amounts of samples to be measured into successive cells;

reagent delivery means for delivering given amounts of a reagent into successive cells;

means for projecting a light beam into successive cells;

means for detecting light scattered by particles included in a reaction liquid contained in a cell to generate a photoelectric signal; and

means for receiving the photoelectric signal and measuring the antigen-antibody reaction in accordance with a power spectrum density of fluctuation in intensity of the scattered light.

2. An analyzer according to claim 1, wherein said reagent delivery means is arranged at an upstream position with respect to the sample delivery means viewed in the feeding direction of cells, and the analyzer further comprises means for projecting a light beam into a cell in which the reagent has been delivered, means for receiving light scattered by particles contained in the reagent to generate a second photoelectric signal, and means for receiving the second photoelectric signal to derive a power spectrum density of fluctuation in intensity of the scattered light prior to the antigen-antibody reaction.

3. An analyzer according to claim 1, wherein said feeding means comprises an endless belt rotated in a vertical plane.

4. An analyzer according to claim 1, further comprising a cell loader for automatically supplying cells into the feeding means, and a cell unloader for automatically removing cells from the feeding means.

5. An analyzer according to claim 1, wherein said feeding means comprises a turntable along whose periphery are arranged a number of cells, and means for rotating the turntable in a stepwise manner.

6. An analyzer according to claim 5, wherein said light beam projecting means and detecting means are so arranged that a photometry is effected for a cell which situates immediately after a position at which the reagent containing particles is delivered.

7. An analyzer according to claim 6, wherein sid sample delivery means is arranged to deliver a sample into a cell for which the photometry is effected.

8. An analyzer according to claim 5, further comprising a liquid-type thermostat arranged underneath the turntable.

9. An analyzer according to claim 8, further comprising an ultrasonic vibrating element secured to an outer surface of the thermostat for applying an ultrasonic wave to the reaction liquids contained in the cells via a liquid of the thermostat.

10. An analyzer according to claim 8, wherein said light beam projecting means comprises a light source arranged outside the thermostat for emitting the light beam and an optical fiber having an incident end arranged in opposite to the light source and an exit end arranged in opposite to a cell within a liquid of the thermostat.

11. An analyzer according to claim 8, wherein said scattered light detecting means comprises an optical fiber having an incident end arranged within a liquid of the thermostat and an exit end arranged outside the thermostat and a photodetector arranged in opposite to the exit end of the optical fiber.

12. An analyzer according to claim 1, wherein said light beam projecting means comprises a light source for emitting the light beam, and an optical system including a movable reflecting mirror for reflecting the light beam toward two adjacent stop positions of the turntable so that the light beam is projected alternately into two cells indexed at said adjacent stop positions.

13. An analyzer according to claim 12, wherein said scattered light detecting means comprises a collimator having two incident openings arranged in opposite to said two cells indexed at the adjacent stop positions and an exit opening, and a photodetector arranged in opposite to the exit opening.

14. An analyzer according to claim 13, wherein said reagent delivery means is so arranged that the reagent containing particles is delivered into a cell indexed at one of said two adjacent stop positions which is downstream viewed in the rotational direction of the turntable.

15. An analyzer according to claim 14, wherein said sample delivery means is so arranged that a sample is delivered into a cell indexed at a stop position immediately after said one of two adjacent stop positions.