Detector assembly for analyzer instrument

• US 4,826,660 A
• Filed: 05/07/1987
• Issued: 05/02/1989
• Est. Priority Date: 05/07/1987
• Status: Expired due to Term

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Smith, Randall W., Smith, Roger E.
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US07/046,953
• Time in Patent Office: 726 Days
• Field of Search: 356/244, 356/246, 356/418, 356/419, 356/440, 422/63-67, 422/68
• US Class Current: 422/82.05
• CPC Class Codes: G01N 21/255 Details, e.g. use of specially adapted sources, lighting or optical systems
• CPC Class Codes: G01N 2201/04 Batch operation; multisample devices

Abstract

A detector assembly for use with an analyzer instrument comprises a light source for providing a light beam for analyzing one or more substances in a sample. A detector is included for detecting light associated with the sample under analysis. An analysis station permits the light beam to enter the sample and shields the sample from extraneous light. The analysis station further permits light to pass from the sample to the light detector. A plurality of filters is selectively positionable in the light beam between the light source and the sample. These filters permit different wavelengths of light to pass therethrough so that selective light analysis of the sample may be performed.

Claims

1. A detector assembly suitable for use in an instrument for analyzing samples carried in a light transmissive carrier comprising:

a light source for providing a light beam for use in analyzing one or more substances in a sample;

a detector for detecting light associated with the sample under analysis;

an analysis station into which a carrier for the sample to be analyzed is positionable and into which the light beam is to be directed, said analysis station including a body membrane with a central opening for receiving the carrier and for surrounding same to shield the sample from extraneous light and to maintain the position of the carrier during analysis, said station including a first aperture in communication with said central opening for permitting incident light from the beam to enter the carrier with the sample, and a second aperture in communication with said central opening for permitting light to pass from the sample to said detector so that the sample may be analyzed;

a coating on said body member around the central opening to maximize the light shielding of the sample from extraneous light; and

a rotatable filter wheel having a plurality of light filters spaced in a substantially circular arrangement in said wheel, each filter being interposable in the light beam between the light source and the analysis station, the filters permitting different wavelengths of light to pass therethrough so that selective light analysis of the sample may be performed depending upon the position of the wheel.

2. The assembly of claim 1 which further includes a first lens for focusing the light beam from said source into the analysis station.

3. The assembly of claim 2 which further includes a second lens for focusing the light from the sample into the detector.

4. The assembly of claim 1 wherein said central opening is a substantially cylindrical bore for surrounding a substantially cylindrical carrier which carries the sample for analysis.

5. The assembly of claim 4 wherein said body member is movable between a first position at which the central opening surrounds the carrier and a second position out of surrounding association with said carrier to facilitate the passage of the carrier into and out of said analysis station.

6. The assembly of claim 5 which further includes means for operatively moving said body member between the first and second positions to accommodate the receipt of the carrier into the analysis station.

7. The assembly of claim 1 wherein the included angle between the first and second apertures is substantially 180°

.

8. The assembly of claim 1 wherein the included angle between the first and second apertures is substantially 90°

.

9. The assembly of claim 8 which further includes a third aperture in communication with said central opening for permitting light to pass from said sample to said detector, wherein the included angle between the first and third apertures is substantially 180°

.

10. The assembly of claim 1 wherein said filters are removably positioned in said wheel so that the filters may be removed without dissembling the wheel from the assembly.

11. The assembly of claim 1 which further includes means for rotating said wheel so that the filters are selectively positionable in the light beam between said light source and said analysis station.

12. The assembly of claim 1 wherein each filter is positioned in said wheel at an angle substantially at 45°

with respect to the plane of rotation of said wheel.

13. The assembly of claim 12 wherein the filters are arranged in aligned and opposed pairs so that the included angle between the faces of each pair of filters is substantially 90°

.

14. The assembly of claim 13 wherein one filter of each pair is interposed in the light beam between the light source and the analysis station and the other filter of each pair is interposed in the light path between the sample and the detector.

15. The assembly of claim 14 wherein each pair of filters is selected so that the first filter of the pair passes light at a wavelength to excite a fluorochrome associated with the sample under analysis and the second filter of the pair passes light at a wavelength of emission from said fluorochrome.

16. The assembly of claim 1 wherein said detector is selected to receive fluorescent emissions from said sample.

17. The assembly of claim 1 wherein said detector is selected to count photons emitted from said sample.

18. The assembly of claim 1 wherein said detector is selected to receive light scattered by said sample.

19. The assembly of claim 1 wherein said detector is selected to measure light absorbed by said sample.

20. A detector assembly for use with an analyzer instrument comprising:

means for providing a light beam for analyzing one or more substances in a sample;

means for detecting light associated with said sample under analysis;

means for permitting said light beam to enter said sample and for shielding said sample from extraneous light and for maintaining the position of the sample during analysis, said permitting means further permitting light to pass from said sample to said means for detecting;

a coating on a surface associated with said permitting and shielding means to maximize the light shielding of the sample from extraneous light; and

a plurality of filters selectively positionable in the light beam between the means for providing light and the sample, the filters permitting different wavelengths of light to pass therethrough so that selective light analysis of the sample may be performed.

21. A detector assembly for use in an instrument for analyzing samples carried in a light transmissive tube comprising:

a light source for providing an incident light beam for use in analyzing one or more substances in a sample;

a detector for detecting light associated with the sample under analysis;

an analysis station including a movable body member with a substantially cylindrical bore into which a tube carrying the sample to be analyzed is positionable, said body member surrounding the tube when positioned in the bore to shield the sample from extraneous light to maintain the position of the tube during analysis, said body member including a first aperture in communication with said bore for permitting incident light to enter the tube with the sample and a second aperture in communication with said bore for permitting light to pass from said sample to said detector so that the sample may be analyzed, said body member being operatively movable between a first position at which the bore surrounds the tube and a second position out of surrounding association with the tube to facilitate the passage of the tube into and out of the analysis station;

a coating on said body member around the central opening to maximize the light shielding of the sample from extraneous light;

a rotatable filter wheel having a plurality of removably mounted light filters spaced in a substantially circular arrangement in said wheel, each filter being interposable in the light beam between the light source and the analysis station, the filters permitting different wavelengths of light to pass therethrough so that selective light analysis of the sample may be performed depending upon the position of the filter wheel; and

means for rotating said wheel so that each filter is selectively positionable in the light beam between the light source and the analysis station.