Multiwell plates containing membrane inserts

• US 4,828,386 A
• Filed: 06/19/1987
• Issued: 05/09/1989
• Est. Priority Date: 06/19/1987
• Status: Expired due to Term

• Current Assignee: Pall Corporation (Danaher Corporation)
• Original Assignee: Pall Corporation (Danaher Corporation)
• Inventors: Matkovich, Vlado I., Degen, Peter J.
• Primary Examiner(s): McGraw, Vincent P.
• Application Number:
  US07/064,342
• Time in Patent Office: 690 Days
• Field of Search: 356/246, 436/524, 436/531, 436/809
• US Class Current: 356/246
• CPC Class Codes: B01L 2200/16 Reagents, handling or storing thereof
• CPC Class Codes: G01N 2021/0325 Cells for testing reactions, e.g. containing reagents
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/03 Cuvette constructions
• CPC Class Codes: G01N 2201/0446 Multicell plate, sequential
• CPC Class Codes: G01N 33/5304 Reaction vessels, e.g. agglutination plates for solid-phase systems G01N33/543
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays

Abstract

A multiwell plate suitable for use in a spectrometer which uses a vertical beam of light comprising a first plate having a plurality of wells for receiving sample, wherein the wells have transparent bottom surfaces to allow for the transmission of a vertical beam of light, and a unitary insert comprising a biochemically compatible microporous surface capable of binding biological materials shaped to fit into at least one well of the plate without interfering with the vertical beam of light.

Claims

1. A multiwell plate suitable for use in a spectrophotometer which uses a vertical beam of light comprising:

a first plate having a plurality of wells for receiving sample, wherein said wells have transparent bottom surfaces to allow for the transmission of a vertical beam of light;

a unitary insert comprising a biochemically compatible microporous binding surface shaped to fit into at least one well of said plate without interfering with said vertical beam of light so as to provide a free light path through said well, said free light path passing through said sample when said sample is present in said well.

2. The multiwell plate of claim 1, wherein said insert comprises a second plate arranged to fit over said first plate and having one or more projections which fit into said one or more of said wells.

3. The multiwell plate of claim 2, wherein said projections comprise hollow cylinders with open top and bottom surfaces.

4. The multiwell plate of claim 2, wherein said projections comprise cylinders with a top or bottom surface through which said beam of light is transmitted substantially without loss.

5. The multiwell plate of claim 2, wherein said insert is removable.

6. The multiwell plate of claim 2, wherein said second plate locks to said first plate when said projection is fully inserted into said well.

7. The multiwell plate of claim 2, wherein said insert comprises a sufficient number of projections to fit into all of said wells.

8. The multiwell plate of claim 2, wherein said insert comprises a single projection which fits into a single well.

9. The multiwell plate of claim 1, wherein said insert fits completely within said well.

10. The multiwell plate of claim 9, wherein said insert comprises a sheet of microporous material longer than the diameter of said well.

11. The multiwell plate of claim 10, wherein said sheet is substantially equal in length to the circumference of said well and said sheet fits against the vertical walls of said well.

12. The multiwell plate of claim 9, wherein said insert comprises a hollow polyhedron without a top or bottom surface.

13. The multiwell plate of claim 12, wherein said polyhedron is a triangular prism.

14. The multiwell plate of claim 9 wherein said insert fits within the bottom two-thirds of said well.

15. The multiwell plate of claim 14, wherein said well comprises substantially vertical walls and said vertical walls comprise an inward projection above the height of said insert, whereby said insert is locked into position in said well.

16. The multiwell plate of claim 9, wherein said microporous surface comprises polyamide affixed to a flexible backing sheet.

17. The multiwell plate of claim 16, wherein said backing sheet is resilient.

18. The multiwell plate of claim 9, wherein said insert is irremovably attached to said well.

19. The multiwell plate of claim 9, wherein said insert is removably located within said well.

20. The multiwell plate of claim 1, wherein said microporous surface comprises polyamide.