Continuous flow type homogenizer

• US 4,828,395 A
• Filed: 12/13/1985
• Issued: 05/09/1989
• Est. Priority Date: 02/21/1985
• Status: Expired due to Term

• Current Assignee: Yamato Scientific Co., Ltd.
• Original Assignee: Yamato Scientific Co., Ltd.
• Inventors: Saito, Tatsuhiko, Ida, Masahiro, Orii, Takeshi, Takabatake, Hikaru, Mori, Hiroaki
• Primary Examiner(s): Coe, Philip R.
• Assistant Examiner(s): Machuga, Joseph S.
• Application Number:
  US06/809,094
• Time in Patent Office: 1,243 Days
• Field of Search: 366/143, 366/149, 366/286, 366/331, 366/349, 366/601, 366/279, 366/285, 366/209, 366/1, 241/2, 241/246, 241/169.1, 241/169.2, 241/101.7, 241/65, 241/66, 241/258, 241/259, 215/13 R, 141/97, 318/384, 16/DIG. 39, 16/DIG. 40, 192/110 S, 285/902
• US Class Current: 366/143
• CPC Class Codes: C12M 47/06 Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material lysis of microorganisms C12N1/06; extracting or separating nucleic acids from biological samples C12N15/1003
• CPC Class Codes: C12N 1/066 by physical methods
• CPC Class Codes: Y10S 366/601 Motor control
• CPC Class Codes: Y10S 388/903 Protective, e.g. voltage or current limit
• CPC Class Codes: Y10S 388/907 Specific control circuit element or device
• CPC Class Codes: Y10S 388/915 Sawtooth or ramp waveform generator

Abstract

There is provided a continuous flow type homogenizer. The homogenizer comprises, a tubular container having at upper end thereof an inlet for introducing tissue to be homogenized and at lower end thereof an outlet for discharging the homogenized tissue, a pestle disposed freely rotatable in the tubular container with a clearance defined between the inner surface of the tubular container and the peripheral surface of the pestle, a rotation shaft driving means connected to a rotation shaft of the pestle, a speed setting means for setting the rotation speed of the pestle, and a speed controlling means for controlling the speed of the rotation shaft with a predetermined speed gradient with respect to a speed value set by the speed setting means. '"'"'

Claims

1. A continuous flow-type homogenizer, comprising:

a tubular container having an inner surface and at an upper end of the container an open inlet for the continuous introduction of tissue to be homogenized and, at a lower end thereof, an outlet for discharging the homogenized tissue;

a pestle having a peripheral surface including an upper portion and a middle portion and being disposed freely rotatable in said tubular container with a clearance defined between said inner surface of said tubular container and the peripheral surface of said pestle, and said pestle further defining a plurality of grooves in the peripheral surface thereof for feeding the introduced tissue toward the outlet of said tubular container, said grooves extending from said upper portion of said peripheral surface into the middle portion thereof, each of said grooves having a gradient of depth along its length such that it becomes gradually shallower as it proceeds from said upper portion toward and into said middle portion;

a rotation shaft connected to said pestle;

a rotation shaft driving means connected to the rotation shaft connected to said pestle;

a speed setting means for setting the rotation speed of said pestle according to the kind of tissue to be homogenized; and

,

a speed controlling means for controlling the acceleration/deceleration of said rotation shaft with a predetermined speed gradient with respect to a speed value set by said speed setting means during all periods of change of speed of said pestle, wherein said predetermined speed gradient is set such that the rotational speed of the pestle is gently accelerated when rotation of the pestle is started and the rotational speed is gently decelerated when the rotation is stopped and also such that the rotational speed is gently accelerated or decelerated when the rotational speed of the pestle is changed during the operation of the homogenizer so as to assure accurate and repeatable tissue homogenization conditions within said tubular container with reduced risk of homogenizer failure and/or tissue scattering from the open inlet of the container into the environment external to the container.

2. A continuous flow type homogenizer of claim 1, further comprising a one-way clutch through which a driving force is transmitted from said rotation shaft driving means to said rotation shaft.

3. A continuous flow type homogenizer of claim 1, further comprising a cap provided for the tissue introducing inlet of said tubular container.

4. A continuous flow type homogenizer of claim 1, further comprising a cooling means arranged outside said tubular container.

5. A continuous flow type homogenizer of claim 1, further comprising an adjusting means for adjusting the relative height of said pestle with respect to said tubular container.

6. A continuous flow type homogenizer of claim 5, wherein the inner surface of said tubular container is tapered, said inner surface facing to the outer surface of said pestle to form a clearance therebetween to carry out the cell disruption.

7. The continuous flow type homogenizer of claim 6 wherein the outer surface of the pestle is tapered with a gradient similar to that of the taper of the inner surface of the tubular container.

8. A continuous flow type homogenizer of claim 5, wherein said adjusting means comprises a means which moves up and down on the rotation shaft.

9. The continuous flow type homogenizer of claim 5, wherein the adjusting means comprises means for releasably gripping the rotating shaft.

10. The continuous flow type homogenizer of claim 9, wherein the gripping means comprises a spring means that clamps onto the rotation shaft when compressed.

11. A continuous flow type homogenizer of claim 1, further comprising a safety cover which is freely fitted to and removeable from said tubular container.

12. A continuous flow type homogenizer of claim 11, wherein said cover is at least partly transparent so that, the homogenizing process performed in said homogenizer may be observed through said cover.

13. The continuous flow type homogenizer of claim 11, wherein the safety cover comprises a transparent flexible material in a cylindrical shape having an inner diameter smaller than the outer diameter of the tubular container and further having a longitudinal cut so that the cover may be expanded to fit over the container.

14. The continuous flow type homogenizer of claim 1, further comprising a safety cover of transparent flexible material in a cylindrical shape having an inner diameter smaller than the outer diameter of the tubular container and further having a longitudinal cut so that the cover may be expanded to fit over the container.

15. A continuous flow type homogenizer as set forth in claim 1 wherein said rotation shaft is connected to the pestle by means of a resilient coupling.