Apparatus and method for separating phases of blood

• US 4,828,716 A
• Filed: 04/03/1987
• Issued: 05/09/1989
• Est. Priority Date: 04/03/1987
• Status: Expired due to Fees

• Current Assignee: DuPont Canada, Inc. (Corteva, Inc.)
• Original Assignee: Andronic Devices, Ltd.
• Inventors: McEwen, James A., Dance, Mark N., Godolphin, William J., Furse, Martin L., Osborne, John C., Bohl, Rainer M.
• Primary Examiner(s): Fisher, Richard V.
• Assistant Examiner(s): JONES, WILLIAM G
• Application Number:
  US07/033,769
• Time in Patent Office: 767 Days
• Field of Search: 494/7, 494/10, 494/11, 494/19, 494/37, 210/104, 210/143, 210/516, 210/518, 210/739, 210/740, 210/744, 210/745, 210/782, 210/789, 210/512.1, 210/787, 422/102, 422/72, 436/177
• US Class Current: 210/740
• CPC Class Codes: B01L 3/50215 using a float to separate phases
• CPC Class Codes: B01L 9/06 Test-tube stands; Test-tube holders
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

Method and apparatus for partitioning a pre-selected phase of a sample of liquid, such as blood, having a plurality of phases of differing densities the method comprising the steps of introducing a sample into a chamber of constant cross-sectional shape and a volume greater than that of the sample, ordering the phases of the sample concentrically by rotating the chamber about its longitudinal axis, reducing the volume of the chamber in response to a separation control signal, removing a portion of the separated sample in order of phase, deriving information about the portion of the sample being removed from the chamber and modifying the separation control signal in response to the derived information. Also disclosed is an apparatus for separating a pre-selected phase of a sample contained in an enclosed chamber, wherein said apparatus can be used with a separating means which rotates the chamber about either a longitudinal axis of the chamber, or about an axis perpendicular to the longitudinal axis of the chamber and not passing through the chamber. In addition, separating means in which such an enclosed chamber is rotated about a longitudinal axis is described.

Claims

1. A method for partitioning a pre-selected phase of a sample of liquid, comprising the steps of:

containing in an elongated chamber having a substantially constant cross-sectional area a sample of liquid having a plurality of phases of differing densities;

ordering the phases of the sample concentrically by rotating the chamber around its longitudinal axis;

while the phases are ordered, reducing the volume of the chamber in response to a separation control signal by reducing the length of the chamber;

receiving from the chamber in order of phase the portion of the volume of sample which exceeds the volume to which the chamber is reducing;

deriving phase-separation information about the portion of the sample being received from the chamber; and

modifying the separation control signal upon comparison of the derived information with reference information to further reduce, hold constant, or increase the volume of the chamber.

2. A method as described in claim 1 wherein the rotation of the chamber around the longitudinal axis is controllable by a spin control signal, and including the steps of:

producing a spin control signal to control the speed and duration of rotation; and

deriving phase-separation information from the sample and comparing the derived information to stored reference information; and

modifying the spin control signal in response to the results of the comparison between the derived information and stored information.

3. A method for partitioning a pre-selected phase of a liquid sample, comprising the steps of:

establishing a partial vacuum in an elongated chamber having a substantially constant cross-sectional area;

containing in the chamber a sample of liquid having a plurality of phases;

ordering the phases of the sample by rotating the chamber around its longitudinal axis;

while the phases are ordered, reducing the volume of the chamber in response to a separation control signal by reducing the length of the chamber;

receiving from the chamber in order of phase the portion of the sample volume that exceeds the volume to which the chamber is reducing;

deriving phase-identifying information about the portion of the sample being received from the chamber; and

modifying the separation control signal upon comparison of the derived information with reference information to further reduce, hold constant, or increase the volume of the chamber.

4. Apparatus for partitioning a sample of liquid having first and second phase portions of different densities, comprising:

(a) an elongated tubular member for containing the sample; and

(b) a phase separating element disposed within the tubular member and movable therein through the sample, the phase separating element including first and second apart-spaced valves having an intermediate chamber located therebetween, the sample passing through the intermediate chamber when the phase separating element is moved through the liquid, the first and second valves being configured to open when the phase separating element is moved through the sample and to close when the phase separating element is not moved through the sample.

5. The apparatus of claim 4 including control means for sending a first parameter of the sample in the intermediate chamber and for generating a control signal representative of the first sensed parameter, and for varying the movement of the phase separating element in response to the control signal.

6. The apparatus of claim 5 further including sample ordering means controllable for ordering the phases of the sample.

7. The apparatus of claim 6 wherein the control means further includes second sensing means for sensing a second parameter and for generating a control signal representative of the second sensed parameter, and for varying the sample ordering means in response to the second sensed parameter.

8. The apparatus of claim 6 wherein the sample ordering means orders the sample so that the first phase portion is nearer to the longitudinal axis of the tubular member than is the second phase portion.

9. The apparatus of claim 5 wherein the tubular member includes indicia attached thereto, the control means further including indicia sensing means for sensing the indicia attached to the tubular member and for generating an identification signal representative of that indicia.