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Nucleotide analogs for nucleic acid labeling and detection

  • US 4,828,979 A
  • Filed: 11/08/1984
  • Issued: 05/09/1989
  • Est. Priority Date: 11/08/1984
  • Status: Expired due to Term
First Claim
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1. In the processof detecting the presence of a target polynucleotide, said process comprisingincorporating a deoxyribonucleotide, modified by the attachment thereto of a biotin, into a polynucleotide complementary to said target polynucleotide;

  • hybridizing said complementary polynucleotide to said target polynucleotide; and

    detecting the presence of said biotin;

    the improvement comprising using, for said modified deoxyribonucleotide, compound of the form
    
    
    space="preserve" listing-type="equation">(d)BTP--NH--L--X, whereTP represents triphosphate, and (D)BTP represents dATP, dCTP, dGTP, 3'"'"'-deoxy ATP, 3'"'"'-doexy CTP, 3'"'"'-deoxy GTP, 2'"'"',3'"'"'-dideoxy ATP, 2'"'"',3'"'"'-dideoxy CTP or 2'"'"',3'"'"'-dideoxy GTP;

    L is bonded through the N of NH to the N6 position of B when B represents adenosine, to the N4 position of B when B represents cytidine, and to the N2 position of B when B represents guanosine;

    L presents either(CH2)n, where 1≦

    n≦

    12,(CH2)n NH--CO-(CH2)m, where 2≦

    n+m≦

    24,(CH2)n O--CO--(CH2)m, where 2≦

    n+m≦

    24, or(CH2)n S--CO--(CH2)m, where 2≦

    n+m≦

    24; and

    X represents ##STR1## where Y represents either NH, O, or S.

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