Enzymatic neutralization of hydrogen peroxide
First Claim
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1. A method for disinfecting a medical device comprising the steps:
- (a) immersing a medical device in a hydrogen peroxide solution for a time sufficient to disinfect said device,(b) decomposing any residual hydrogen peroxide by use of a catalytically effective amount of a protein capable of decomposing hydrogen peroxide, said protein being immobilized on a composite article, comprising in sequence;
(1) a polymeric fibrous support, wherein said support has been subjected to a surface modification treatment to provide binding sites thereon, said treatment providing a layer of inorganic oxide having a uniform thickness when dried in the range of 2 to 500 nm to provide binding sites thereon, or being a radio frequncy or microwave plasma treatment carried out at a frequency of 10 to 125 kilohertz with a power density in the range of 0.05 to 2.25 w/cm2 generated between two electrodes in a gas at a pressure in the range of 10 mtorr to 10 torr,(2) a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound, and(3) a biological active protein bound to said layer of protein immobilizer compound and capable of decomposing hydrogen peroxide.
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Abstract
A method for disinfecting a medical device comprising the steps of immersing a medical device in a hydrogen peroxide solution for a time sufficient to disinfect said device, decomposing any residual hydrogen peroxide by use of a catalytically effective amount of a protein capable of decomposing hydrogen peroxide, said protein being immobilized on a composite article, said composite article comprising in sequence a support, a layer of protein immobilizer compound, and a biologically active protein. The surface modification treatment is a gelled network of inorganic oxide particles or a plasma treatment.
59 Citations
19 Claims
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1. A method for disinfecting a medical device comprising the steps:
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(a) immersing a medical device in a hydrogen peroxide solution for a time sufficient to disinfect said device, (b) decomposing any residual hydrogen peroxide by use of a catalytically effective amount of a protein capable of decomposing hydrogen peroxide, said protein being immobilized on a composite article, comprising in sequence; (1) a polymeric fibrous support, wherein said support has been subjected to a surface modification treatment to provide binding sites thereon, said treatment providing a layer of inorganic oxide having a uniform thickness when dried in the range of 2 to 500 nm to provide binding sites thereon, or being a radio frequncy or microwave plasma treatment carried out at a frequency of 10 to 125 kilohertz with a power density in the range of 0.05 to 2.25 w/cm2 generated between two electrodes in a gas at a pressure in the range of 10 mtorr to 10 torr, (2) a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound, and (3) a biological active protein bound to said layer of protein immobilizer compound and capable of decomposing hydrogen peroxide. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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15. A method of disinfecting a medical device comprising the steps:
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(a) immersing a medical device in a hydrogen peroxide solution for a time sufficient to disinfect said device, (b) decomposing any residual hydrogen peroxide by use of a catalytically effective amount of catalase, said catalase being immobilized on a composite article, said composite article comprising in sequence; (1) a polymeric woven or nonwoven fibrous support, wherein said support has been sujected to a surface modification treatment to provide binding sites thereon, said treatment providing a layer of inorganic oxide having a uniform thickness when dried in the range of 2 to 500 nm to provide binding sites thereon, or being a radiowave or microwave plasma treatment carried out at a frequency of 10 to 125 kilohertz with a power density in the range of 0.05 to 2.25 w/cm2 generated between two electrodes in a gas at a pressure in the range of 10 mtorr to 10 torr, (2) a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound, and (3) catalase bound to said layer of protein immobilizer compound. - View Dependent Claims (16, 17)
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18. A kit for disinfecting a medical device wherein hydrogen peroxide is the disinfecting agent, said kit comprising in one package an amount of hydrogen peroxide solution sufficient to disinfect said device and a composite article comprising in sequence:
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(1) a polymeric fibrous support, wherein said support has been subjected to a surface modification treatment to provide binding sites thereon, said treatment providing a layer of inorganic oxide having a uniform thickness when dried in the range of 2 to 500 nm to provide binding sites thereon, or being a radiowave or microwave plasma treatment carried out at a frequency of 10 to 125 kilohertz with a power density in the range of 0.05 to 2.25 w/cm2 generated between two electrodes in a gas at a pressure in the range of 10 mtorr to 10 torr, (2) a layer of a protein immobilizer compound comprising a polymer or a silane-functional compound, and (3) a biologically active protein selected from the group consisting of catalase and peroxidase bound to said layer of protein immobilizer compound.
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19. A kit for disinfecting a medical device wherein hydrogen peroxide is the disinfecting agent, said kit comprising the combination of a first package containing an amount of hydrogen peroxide solution sufficient to disinfect said device, and a second package containing a composite article comprising in sequence:
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(1) a polymeric fibrous support, wherein said support has been subjected to a surface modification treatment to provide binding sites thereon, said treatment providing a layer of inorganic oxide having a uniform thickness when dried in the range of 2 to 500 nm to provide binding sites thereon, or being a radiowave or microwave plasma treatment carried out at a frequency of 10 to 125 kilohertz with a power density in the range of 0.05 to 2.25 w/cmhu 2 generated between two electrodes in a gas at a pressure in the range of 10 mtorr to 10 torr, (2) a layer of protein immobilizer compound comprising a polymer or a silane-functional compound, and (3) a biologically active protein selected from the group consisting of catalase and peroxidase bound to said layer of protein immobilizer compound.
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Specification