Pyroelectric enzyme detector

• US 4,829,003 A
• Filed: 08/28/1986
• Issued: 05/09/1989
• Est. Priority Date: 08/28/1986
• Status: Expired due to Fees

• Current Assignee: Lawrence H. Arney Jr
• Original Assignee: Lawrence H. Arney Jr
• Inventors: Arney, Lawrence H. Jr.
• Primary Examiner(s): Naff, David M.
• Application Number:
  US06/901,428
• Time in Patent Office: 985 Days
• Field of Search: 435/3, 435/4, 435/174, 435/176, 435/177, 435/181, 435/817, 422/95, 204/403
• US Class Current: 435/287.1
• CPC Class Codes: C12M 41/32 of substances in solution
• CPC Class Codes: G01N 25/482 concerning the temperature responsive elements measuring temperature or quantity of heat, thermally-sensitive elements G01K; thermoelectric devices H10N10/00, H10N15/00
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A pyroelectric sensor is provided for detecting enzymes or enzymatic substrates in a liquid or gas stream. The sensor contains a laminate formed of a pyroelectric material sandwiched between upper and lower conducting means to act as electrodes. An electronic circuit means is connected to the conducting means to measure thermally induced electric current and an enzyme or enzymatic substrate is immobilized on a surface of one of the conducting means. The laminate is supported by a support means and a plate means rests on an upper surface of the supported laminate. The plate means has a longitudinal flow channel communicating with the upper surface for flow of a gas of liquid, and a recess for accommodating the electronic circuit means. Inlet and outlet means pass through the plate means and communicate with the flow channel.

Claims

1. A pyroelectric sensor for detecting an enzyme or enzymatic substrate in a gas or liquid stream comprising:

(a) a pyroelectirc laminate having an upper and lower surface comprised of a pyroelectric material sandwiched between upper and lower conducting means to act as electrodes for conducting an electrical response from said pyroelectric material;

(b) electronic circuit means connected to said electrodes to measure thermally induced electric current from said pyroelectric material;

(c) a reactant selected from the group consisting of an enzyme and an enzymatic substrate reactable respectively with a corresponding enzyme or substrate to produce a thermally detectable endothermic or exothermic reaction, said reactant being chemically immobilized on a surface on one of said conducting means;

(d) a support means supporting said laminate;

(e) a plate means resting on the upper surface of said supported laminate, said plate means having a longitudinal flow channel for said gas or liquid stream disposed above and communicating with the upper surface of said laminated and said plate and means having a recess communicating with said upper surface for accomodating said electronic circuit means;

(f) an inlet means passing through said plate means communicating with said flow channel near one end thereof; and

(g) an outlet means passing through said plate means communicating with said flow channel near an end opposite said inlet means.

2. A pyroelectric sensor according to claim 1 wherein the reactant is an enzymatic substrate.

3. A pyroelectric sensor according to claim 1 wherein the reactant is an enzyme.

4. A pyroelectric sensor according to claim 1 wherein said support means is a material of high heat capacity and low thermal conductivity.

5. A pyroelectric sensor according to claim 4 wherein said support means is a thermoplastic or thermosetting resinous material.

6. A pyroelectric sensor according to claim 5 wherein said support means material is styrofoam.

7. A pyroelectric sensor according to claim 4 wherein said support means is a glass material.

8. A pyroelectric sensor according to claim 1 wherein said upper and lower conducting means is a metal.

9. A pyroelectric sensor according to claim 1 including a chemical entity in contact with one of said conducting means reactively responsive to reaction products of said group of an enzyme or an enzymatic substrate to provide a detactable thermal signal.