Immunoassay device enclosing matrixes of antibody spots for cell determinations
First Claim
1. An immunoassay device comprising:
- a. a support having substantially planar, unrestricted surface,b. an array of small, closely-spaced, discrete, antibody-coated areas on the support surface;
c. a cover spaced from the support surface and positioned over an array of antibody-coated areas;
the cover being secured to the support surface to provide a single, unrestricted, enclosed chamber,the cover having at least one aperture communicating with the interior of the single chamber and with ambient atmosphere for introducing a liquid sample into the chamber to flow across the planar surface without impediment and into engagement with all of said coated areas regardless of the location of the aperture relative to the planar surface, andat least one additional aperture in the cover also communicating with the interior of the chamber and ambient atmosphere to allow air to escape from the chamber upon introduction of the sample into the chamber.
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0 Petitions
Accused Products
Abstract
An immunoassay device comprising a support which has a substantially planar surface 14 on which is located an array of small, closely-spaced, discrete, antibody coated areas (spots) 17. A cover 16 is spaced from the support surface 14 and is positioned over the array of antibody coated spots 17. The cover is attached to the support surface to provide an enclosed chamber 30. In the cover there is at least one aperture communicating with the interior of the chamber 30 for introducing a liquid sample into the chamber. In addition, there is at least one additional aperture 34 in the cover, which also communicates with the interior of the chamber to allow air to escape upon introduction of the sample into the chamber. When a cell sample is introduced into the chamber it immediately fills the chamber and cells will settle uniformly over the matrix. The antibody-coated spots function as tiny immunoadsorbents for cells bearing antigens recognized by the antibody. The device can be used to determine the proportion of cells in a sample cell population.
107 Citations
36 Claims
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1. An immunoassay device comprising:
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a. a support having substantially planar, unrestricted surface, b. an array of small, closely-spaced, discrete, antibody-coated areas on the support surface; c. a cover spaced from the support surface and positioned over an array of antibody-coated areas; the cover being secured to the support surface to provide a single, unrestricted, enclosed chamber, the cover having at least one aperture communicating with the interior of the single chamber and with ambient atmosphere for introducing a liquid sample into the chamber to flow across the planar surface without impediment and into engagement with all of said coated areas regardless of the location of the aperture relative to the planar surface, and at least one additional aperture in the cover also communicating with the interior of the chamber and ambient atmosphere to allow air to escape from the chamber upon introduction of the sample into the chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
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21. An immunoassay device comprising:
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a. a support having a substantially planar, unrestricted surface, b. an array of small, discrete, closely-spaced, antibody-coated areas on the planar surface; c. walls extending upwardly from the support surface and secured to the surface to form a single rectangular enclosure located at the perimeter of the array of antibody-coated areas and defining the planar unrestricted surface within the walls, and d. a cover mounted on the walls thereby providing a single, unrestricted enclosed chamber, the cover having at least one aperture communicating with the interior of the chamber interior for introduction a sample into the chamber to flow across the planar surface without impediment and into engagement with all of said coated areas, and at least one other aperture also communicating with the chamber interior to permit the escape of air from the chamber when a sample is introduced into the chamber regardless of the location of either aperture relative to the planar surface. - View Dependent Claims (22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35)
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36. An immunoassay device, comprising:
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a. a glass or plastic support having a substantially planar, unrestricted surface; b. two, separate rectangular arrays of antibody-coated dots on the planar surface, the density of antibody in the dots being about 10 μ
g/ml to 20 μ
g/ml;c. walls extending upwardly from the support surface and located at the perimeter of each array of antibody-coated dots and defining the planar, unrestricted surface within the walls; and d. a cover mounted upon the walls thereby forming an enclosed chamber over each array, the cover having a sample injection aperture communicating with the chamber space and a plurality of apertures also communicating with the chamber space to permit air to escape.
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Specification