Agglutination assay
First Claim
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1. A method of detecting the presence or amount of an analyte in a sample, which comprises:
- forming a reaction medium containing (1) a sample;
(2) a plurality of particles having a binding pair member bound to their surfaces; and
(3) an analyte mimic or analyte binding partner bound to a monovalent complementary partner to said binding pair member;
detecting agglutination of said particles in said reaction medium; and
correlating agglutination with presence of said analyte In said sample if said method is a direct assay and with absence of said analyte if said method is a competitive assay.
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Abstract
A method of detecting the presence and/or amount (concentration) of an analyte in a sample is provided by forming a reaction medium containing (1) a sample; (2) a plurality of particles having a binding pair member bound to their surfaces; and (3) a monovalent complementary partner to the binding pair member to which is bound an analyte mimic or analyte binding partner; and detecting the presence or amount of agglutination of the particles in the reaction medium. In some cases a polyvalent receptor capable of binding to the analyte (and to the analyte mimic, if present) is also present in the reaction medium.
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21 Claims
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1. A method of detecting the presence or amount of an analyte in a sample, which comprises:
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forming a reaction medium containing (1) a sample;
(2) a plurality of particles having a binding pair member bound to their surfaces; and
(3) an analyte mimic or analyte binding partner bound to a monovalent complementary partner to said binding pair member;detecting agglutination of said particles in said reaction medium; and correlating agglutination with presence of said analyte In said sample if said method is a direct assay and with absence of said analyte if said method is a competitive assay. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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- 18. A regeant combination for use in the detection of an analyte, comprising (1) a monovalent complementary partner to a member of a complementary binding pair, said complementary partner having bound thereto an analyte mimic or analyte binding partner, and (2) either (a) a polyvalent receptor capable of binding with said analyte and with said analyte mimic or (b) a plurality of particles having said binding pair member bound to their surfaces.
Specification