Apparatus for determining base sequence of nucleic acid

• US 4,830,830 A
• Filed: 02/18/1986
• Issued: 05/16/1989
• Est. Priority Date: 02/25/1985
• Status: Expired due to Fees

• Current Assignee: Hitachi, Ltd.
• Original Assignee: Hitachi, Ltd.
• Inventors: Watanabe, Kenichi, Harada, Yoshinori, Tokita, Jiro, Tamotu, Simada, Kambara, Hideki
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US06/829,823
• Time in Patent Office: 1,183 Days
• Field of Search: 204/182.6, 204/299 R, 356/528, 356/430, 250/562, 250/572, 250/374, 436/94, 422/71, 422/68
• US Class Current: 204/603
• CPC Class Codes: G01N 27/44726 using specific dyes, markers or binding molecules
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

Nucleic acid fragments labelled by radio isotape migrate at speeds that increase as the molecular weights decrease in a migration path served with a potential at both ends thereof. The migration path is constituted by a pipe-shaped gel support such as a glass tube. A ring-shaped scintillator is provided at a middle portion of the migration path to circumscribe the gel support, and whereby the beta rays are detected. A window may be formed in the glass tube at a portion opposed to the scintillator, so that the beta rays are not attenuated by the glass.

Claims

1. An apparatus for determining base sequence of nucleic acid, comprising:

a pipe-shaped gel supoprt which contains a gel and which constitutes a migration path for nucleic acid fragments labeled by radio isotope;

electrode solution layers installed at both ends of said gel support;

a power source for applying potential across said electrode solution layers;

means for photoelectric conversion; and

a ring-shaped radiation detector for detecting radioactive emissions, converting the detected emissions to light and guiding the generated light to said means for photoelectric conversion, said ring-shaped radiation detector being provided to circumscribe said gel support at a middle portion thereof and being intimately contacted thereto or spaced thereform to form a gap therebetween.

2. An apparatus for determining base sequence of nucleic acid according to claim 1, wherein said ring-shaped radiation detector is a plastic scintillator and is covered with a thin aluminum film to shield external light.

3. An apparatus for determining base sequence of nucleic acid according to claim 1, wherein the ring-shaped radioactive ray detector is a ring-shaped plastic scintillator which exhibits a high sensitivity to beta rays, emits fluorescent light and absorbs 1.7 MeV of radio isotope without permitting leakage of beta rays.

4. An apparatus for determining base sequence of nucleic acid according to claim 3, wherein said ring-shaped plastic scintillator is covered with a thin aluminum film to shield external light.

5. An apparatus for determing base sequence of nucleic acid according to claim 1, wherein said migration path is constituted by a plurality of pipe-shaped gel supports and each of said pipe-shaped gel supports is circumscribed at a middle portion thereof with at least one radioactive ray detector having a ring-shaped scintillator.

6. An apparatus for determining base sequence of nucleic acid according to claim 1, wherein said ring-shaped radiation detector is a plastic scintillator.

7. An apparatus for determining base sequence of nucleic acid according to claim 1, wherein said means for photoelectric conversion is a photo-multiplier tube.

8. An apparatus for determining base sequence of nucleic acid according to claim 1, wherein said ring-shaped radiation detector is a plastic scintillator and said means for photoelectric conversion is a photo-multiplier tube.

9. An apparatus for determining base sequence of nucleic acid according to claim 1, wherein said radioactive emissions are beta rays and said ring-shaped radiation detector converts said beta rays into fluorescent light and guides said fluorescent light to said means for photoelectric conversion.

10. An apparatus for determining base sequence of nucleic acid according to claim 9, wherein said means for photoelectric conversion is a photo-multiplier tube.