Pipette and pipetting apparatus

• US 4,830,832 A
• Filed: 12/12/1986
• Issued: 05/16/1989
• Est. Priority Date: 12/17/1985
• Status: Expired due to Term

• Current Assignee: Hamilton Bonaduz AG (Hamilton Company)
• Original Assignee: Hamilton Bonaduz AG (Hamilton Company)
• Inventors: Arpagaus, Renato, Leyssens, Herwig
• Primary Examiner(s): NOT, DEFINED
• Application Number:
  US06/941,088
• Time in Patent Office: 886 Days
• Field of Search: 422/65, 422/64, 422/63, 422/100, 73/864.13, 73/864.14, 73/864.16, 73/864.17, 73/863.32
• US Class Current: 422/65
• CPC Class Codes: B01L 3/0217 of the plunger pump type medical syringes A61M
• CPC Class Codes: B01L 3/0279 co-operating with positive ejection means
• CPC Class Codes: G01N 2035/041 lifting items out of a rack for access
• CPC Class Codes: G01N 2035/1025 Fluid level sensing
• CPC Class Codes: G01N 2035/103 using disposable tips
• CPC Class Codes: G01N 2035/1069 by adjusting the spacing between multiple probes of a single transferring head
• CPC Class Codes: G01N 35/1067 for transfer to or from containers having different spacing
• CPC Class Codes: G01N 35/1072 with provision for selective pipetting of individual channels
• CPC Class Codes: G01N 35/1083 with one horizontal degree of freedom

Abstract

A pipette includes a pipette body made of plastic material and a pipette piston made of plastic material that is also electrically conductive. The pipette is automatically received or discarded in a pickup device which has an electric transmission filament. A pipetting apparatus has a pickup device for receiving test containers, a pickup section for a microstandard plate, a pipette hopper, and a dosing device. For automatic pipetting, the dosing device receives the pipettes, conveys the pipettes while simultaneously changing the distance of the pipettes to the test containers, absorbs liquid through the pipette, conveys the pipettes while simultaneously changing the distance of the pipette to the microstandard plate, discharges the liquid, and finally ejects the pipette.

Claims

1. A pipetting apparatus comprising:

a plurality of pipettes each having a pipette body having a first end section and;

a pipette piston positioned within the pipette body having a second end section;

the first and second end sections of the pipette body and the pipette piston forming a suction tip with substantially no clearance between the first and second end sections;

a portion of the second end section of the pipette piston extending beyond the first end section of the pipette body in a final position;

at least one of the pipette body and the pipette piston being electrically conductive,

a pickup devices each including a first assembly for receiving the pipette body and a second assembly for receiving the pipette piston;

means for moving the first and second assemblies; and

at least one of the first and second assemblies being electrically conductive;

a first receiving device for receiving a plurality of test containers;

a second receiving device for receiving at least one assembly with vessels, which are arranged in a geometrically regular pattern;

a third feeding device for feeding pipettes from a pipette hopper to the pickup devices;

said first, second and third devices being fixedly arranged adjacent each other in a chassis and accessible from above;

a fourth dosing device provided with the pickup devices and movably disposed above said chassis in order to pick up pipettes in said third feeding device by means of the pickup devices, to absorb liquid from the containers in said first receiving device, and to discharge the absorbed liquid from the pipettes on said at least one assembly and then to eject the pipettes, said fourth device having one support for each pickup device, said supports being arranged side by side in a row, and adjusting means for proportionally adjusting the distance between said supports.

2. An apparatus according to claim 1, wherein said adjusting means includes a roller provided with a number of grooves and a drive unit for said roller, said grooves being helical in shape, each having a different inclination, and the initial and terminal points of said grooves are in a line that extends parallel with the axis of said roller, and said supports have attachments that mesh in said grooves.

3. An apparatus according to claim 1, wherein said first receiving device has a control unit including detectors formed in the pipettes to individually adjust the liquid level in the test containers to the same height as said fourth device.

4. An apparatus according to claim 3, wherein said first device has supports for the test containers and a drive means connected with said control unit and adapted to be operatively connected with said supports in order to individually move the test containers relative to said fourth device.

5. An apparatus according to claim 1, wherein said pipettes are arranged in a support element, and said third feeding device has a conveyor to transport said support elements with the pipettes from the pipette hopper, a displacement device to eject the pipettes from said support element, and a discharge means to prepare the pipettes for being received by the pickup devices.

6. An apparatus according to claim 5, wherein said displacement device and said discharge device respectively contain a drive controllable in a manner such that said displacement device selectively discharges pipettes in said discharge device.

7. An apparatus according to claim 1, wherein each support has a first connecting element to fasten said first assembly of a pickup device, and said fourth device contains an adjusting device that has second connecting elements to fasten said second assembly of of pickup device, and a drive unit to move said second connecting elements relative to said first connecting elements.

8. An apparatus according to claim 7, wherein a first and a second insulating element are respectively disposed between said first and said second connecting elements and said first and said second assemblies of the pickup device, in order to electrically insulate the pickup device with respect to said support.

9. A pipetting apparatus comprising:

a plurality of pipettes each having a pipette body having a first and end section and

a pipette piston positioned within the pipette body having a second end section;

the first and second end sections of the pipette body and the pipette piston forming a suction tip with substantially no clearance between the first and second end sections;

a portion of the second end section of the pipette piston extending beyond the first end section of the pipette body in a final position;

at least one of the pipette body and the pipette piston being electrically conductive;

a pickup devices each including a first assembly for receiving the pipette body and a second assembly for receiving the pipette piston;

means for moving the first and second assemblies; and

at least one of the first and second assemblies being electrically conductive;

a first receiving device for receiving a plurality of individual test containers;

a second receiving device for receiving at least one assembly with vessels, which are arranged in a geometrically regular pattern;

a third feeding device for feeding pipettes from a pipette hopper to the pickup devices;

said first, second and third devices being fixedly arranged adjacent each other in a chassis and accessible from above;

a fourth closing device provided with the pickup devices and movably disposed above said chassis in order to pick up pipettes in said third feeding device by means of the pickup devices, to absorb liquid from the containers in said first receiving device, and to discharge the absorbed liquid from the pipettes on said at least one assembly and then to eject the pipettes, said fourth device being situated in a housing that is guided on one side therof, said housing having a drive unit to move said fourth device above said chassis, said fourth device having one support for each pickup device and adjusting means for proportionally adjusting the distance between the pickup supports; and

said first device having supports for the test containers and drive means operatively connected to the supports for individually moving the test containers relative to the fourth device.