Method of using resonance raman spectroscopy for detection of malignancy disease

• US 4,832,483 A
• Filed: 09/03/1987
• Issued: 05/23/1989
• Est. Priority Date: 09/03/1987
• Status: Expired due to Fees

• Current Assignee: New England Medical Center Hospitals, Inc.
• Original Assignee: New England Medical Center Hospitals, Inc.
• Inventors: Verma, Surendra P.
• Primary Examiner(s): Rosenberger, Richard A.
• Application Number:
  US07/092,424
• Time in Patent Office: 628 Days
• Field of Search: 356/39, 356/301
• US Class Current: 356/39
• CPC Class Codes: G01N 21/65 Raman scattering
• CPC Class Codes: G01N 33/574 for cancer

Abstract

A method for determining the presence of malignancy disease in a subject person includes obtaining a test sample of blood plasma from the person, measuring intensities of Raman scattering from the plasma at frequencies in the frequency shift range between 1000 cm^-1 and 1600 cm^-1, and comparing the intensities to intensities of Raman scattering, at comparable frequencies, from normal human plasma.

Claims

1. A method for determining the presence of malignancy disease in a subject person, comprising

obtaining a test sample of blood plasma from the person, and

measuring intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1000 cm^-1 and 1600 cm^-1,

a substantial difference between said intensities and intensities of Raman scattering, at comparable frequencies, from normal human plasma indicating a presence of malignancy disease in the person.

2. The method of claim 1 wherein said method involves recording a plurality of said intensities of said test sample and comparing said plurality of recorded intensities to a plurality of intensities, at comparable frequencies, of said normal plasma.

3. A method for detecting the presence of malignancy disease in a subject person, comprising

obtaining a test sample of blood plasma from the person,

recording intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1000 cm^-1 and 1600 cm^-1, and

determining from said recording a frequency at which the pseudo-Raman band reaches a maximum in said frequency shift range,

a said pseudo-Raman band maximum at a frequency in the frequency shift range between 1000 cm^-1 and 1350 cm^-1 indicating a presence of malignancy disease in the person, and a said pseudo-Raman band maximum at a frequency in the frequency shift range between 1350 cm^-1 and 1600 cm^-1 indicating an absence of malignancy disease in the person.

4. The method of claim 3, a said pseudo-Raman band maximum at a frequency in the frequency shift range between 1160 cm^-1 and 1250 cm^-1 indicating a presence of malignancy disease in the person, and a said pseudo-Raman band maximum at a frequency in the frequency shift range between 1450 cm^-1 and 1530 cm^-1 indicating an absence of malignancy disease in the person.

8. The method of claim 3, 5, 6, or 7 wherein said intensities of Raman scattering are recorded at regular intervals over at least a portion of said frequency shift range.

9. The method of claim 8 wherein said intervals are no greater than 100 cm^-1.

10. The method of claim 8 wherein said intervals are no greater than 50 cm^-1.

11. The method of claim 8 wherein said intervals are sufficiently small that together said recorded intensities comprise a substantially continuous spectrograph over said portion of said frequency shift range.

5. A method for detecting the presence of malignancy disease in a subject person, comprising

obtaining a test sample of blood plasma from the person,

recording intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1000 cm^-1 and 1600 cm^-1,

determining from said recording the height, I₁₅₃₀, of the 1530 cm^-1 peak at its maximum intensity, and

determining from said recording the height of the 1160 cm^-1 peak at its maximum intensity, I₁₁₆₀,

a said ratio I₁₅₃₀ /I₁₁₆₀ in the range between 1.30 and 1.59 indicating a presence of malignancy disease in the person, and a said ratio I₁₅₃₀ /I₁₁₆₀ in the range between 1.00 and 1.20 indicating an absence of malignancy disease in the person.

6. A method for detecting the presence of malignancy disease in a subject person, comprising

obtaining a test sample of blood plasma from the person, and

recording intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1400 cm^-1 and 1600 cm^-1,

a substantial difference between said intensities and intensities of Raman scattering, at comparable frequencies, from normal human plasma indicating a presence of malignancy disease in the person.

7. A method for detecting the presence of malignancy disease in a subject person, comprising

obtaining a test sample of blood plasma from the person,

recording intensities of Raman scattering from said plasma at frequencies in the frequency shift range between 1500 cm^-1 and 1560 cm^-1,

determining from said recording the shape of that portion of the 1530 cm^-1 band between 1500 cm^-1 and 1560 cm^-1, and

measuring the half-height band width of said 1530 cm^-1 band,

a said half-height band width of 16.5±

2.6 cm^-1 indicating a presence of malignancy disease in the person, and a said half-height band width of 22±

2.5 cm^-1 indicating an absence of malignancy disease in the person.