Electrofusion cell and method of making the same
First Claim
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1. An apparatus for fusing cells comprising:
- a laminate formed by a first planar membera grid of opposed interdigitated electrodes coated on the first member,a second planar member,a photopolymer, that is resistant to water, having a high dielectric, sandwiched between the first and second members, the photopolymer and members defining a cavity encompassing at least a portion of the opposed electrodes, means for applying electrical signals to the electrodes, andmeans in the second member defining a pair of ports for admitting liquids and cells to the chamber.
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Abstract
An apparatus and method of use is provided for the fusion of different cell types into hybrid cells through the use of electrofusion. The apparatus consists of thin-film electrodes etched onto an optically clear lower plate, a channel defined at its boundaries by a photocopolymer material laminated between the lower plate and an upper optically clear top plate providing closure of the channel as well as entry for the cells through the use of integral Luer ports. The apparatus may be produced, with minor adjustment, by using phototools.
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Citations
16 Claims
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1. An apparatus for fusing cells comprising:
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a laminate formed by a first planar member a grid of opposed interdigitated electrodes coated on the first member, a second planar member, a photopolymer, that is resistant to water, having a high dielectric, sandwiched between the first and second members, the photopolymer and members defining a cavity encompassing at least a portion of the opposed electrodes, means for applying electrical signals to the electrodes, and means in the second member defining a pair of ports for admitting liquids and cells to the chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16)
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Specification