Method for enhancing analysis timing in kinetic nephelometry
First Claim
1. A method of analyzing chemical reactions, comprising the steps of:
- (a) producing a rate signal from light scattered by a precipitate formed by the reaction;
(b) measuring the peak value of the rate signal; and
(c) verifying the peak value for a time interval that is a function of the reaction rate.
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Accused Products
Abstract
The peak verify time for kinetic nephelometric measurements of reactions between antigens and antibodies is adjusted as a function of the magnitude of the peak rate order to reduce the time required for peak verification. The scatter signal is zeroed following the end of the peak verification period and the reaction is tested for antigen excess. Reactions during the antigen excess check having rates that exceed a threshold value are accepted as being valid, and no additional measurements are made for such samples. Reactions during the antigen excess check having rates that are less than the threshold value are rejected as being in antigen excess. Samples found to be in antigen excess are diluted and then reanalyzed.
23 Citations
20 Claims
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1. A method of analyzing chemical reactions, comprising the steps of:
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(a) producing a rate signal from light scattered by a precipitate formed by the reaction; (b) measuring the peak value of the rate signal; and (c) verifying the peak value for a time interval that is a function of the reaction rate. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method of analyzing chemical reactions, comprising the steps of:
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(a) producing a scatter signal from light scattered by a precipitate formed by the reaction; (b) measuring the peak rate of change of the scatter signal; (c) verifying the peak rate; (d) testing the reaction to determine whether a selected reagent is in excess; and (e) terminating measurements for reactions in which the reagent is not in excess. - View Dependent Claims (10, 11, 12, 13, 14)
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15. A method of chemical analysis of reactions between antigens and antibodies using kinetic nephelometry, comprising the steps of:
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(a) injecting a sample of an antigen into a reaction vessel; (b) injecting an antibody corresponding to the antigen into the reaction vessel so that the antigen and antibody react to form a precipitate; (c) applying a light beam to the precipitate; (d) producing a scatter signal from light scattered by the precipitate; (e) determining a trial peak rate of change of the scatter signal; (f) testing the trail peak rate to determine whether it is the actual peak rate; (g) varying the time interval for testing the trial peak rate as a function of the trial rate; (h) zeroing the rate signal after veryifying the actual peak rate of the reaction; (i) testing the reaction to determine whether it is in antigen excess; (j) terminating measurements for reactions not in antigen excess; and (k) diluting samples found to be in antigen excess and repeating steps (a)-(j) above until a reaction rate measurement is obtained for a sample that is not in antigen excess. - View Dependent Claims (16, 17, 18)
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19. A method of analyzing chemical reactions, comprising the steps of:
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(a) producing a scatter signal from light scattered by a precipitate formed by the reaction; (b) measuring the peak rate of change of the scatter signal; (c) verifying the peak rate; and (d) zeroing the scatter rate after verifying the actual peak rate of the reaction. - View Dependent Claims (20)
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Specification