Method and apparatus to monitor cholesterol levels with photon correlation spectroscopy

• US 4,836,207 A
• Filed: 09/09/1987
• Issued: 06/06/1989
• Est. Priority Date: 09/09/1987
• Status: Expired due to Fees

• Current Assignee: BETH Israel Medical Center (Mount Sinai Beth Israel)
• Original Assignee: BETH Israel Medical Center (Mount Sinai Beth Israel)
• Inventors: Serur, Juan R., Bursell, Sven-Erik
• Primary Examiner(s): Jaworski, Francis
• Assistant Examiner(s): Hanley, John C.
• Application Number:
  US07/095,052
• Time in Patent Office: 636 Days
• Field of Search: 128/745, 128/633, 128/664, 128/665, 128/666
• US Class Current: 600/318
• CPC Class Codes: A61B 3/1225 using coherent radiation
• CPC Class Codes: A61B 3/135 Slit-lamp microscopes

Abstract

A method to determine the cholesterol level in a patient in a non-invasive manner by delivering a beam of light to a patient'"'"'s eye. The intensity of light scattered from the aqueous of the eye and the mobility of the protein scatterers in the eye are measured and compared to a control.

An apparatus to detect the cholesterol level in a patient in a non-invasive manner which delivers a beam of light to a patient'"'"'s eye, collects the scattered light from the patient'"'"'s eye and correlates the scattered light to a cholesterol level measurement.

Claims

1. Apparatus for monitoring serum cholesterol levels in a non-invasive manner comprising:

(a) a slit lamp biomicroscope including an illumination optical system comprising a laser beam, an attenuator for reducing the power of said laser beam, and a microscope objective lens for focusing said laser beam onto an optical fiber for delivering said laser beam to said slit lamp biomicroscope;

(b) illumination optics mounted on said slit lamp biomicroscope such that light from said laser beam is backscattered from a focal point in the aqueous of a patient'"'"'s eye and collected through a collecting fiber optic at an approximately 130% angle with said laser beam;

(c) a photon counting photomultiplier (PMT) for detecting said backscattered light;

(d) a preamplifier discriminator for converting said backscattered light from said PMT to a digital photopulse output;

(e) a digital autocorrelator for measuring temporal time dependent fluctuations and scattered light intensity in said digital photopulse output;

(f) a means for normalizing scattered light intensity from the aqueous with respect to scattered intensity measured from the lens of a patient'"'"'s eye and means for correlating said normalized scattered light intensity measurement to a serum cholesterol level wherein a patient'"'"'s serum cholesterol level is measured b correlating a normalized scattered light intensity from the aqueous of the patient'"'"'s eye to a serum cholesterol level.

2. A method for monitoring serum cholesterol levels in a non-invasive manner comprising the steps of:

(a) directing a low power laser beam to the aqueous of a patient'"'"'s eye for scattering by molecules in the aqueous of the patient'"'"'s eye;

(b) receiving a backscattered laser light from the aqueous and converting the backscattered light into an electrical signal;

(c) measuring time dependent fluctuations of said signal and calculating the autocorrelation function associated with said fluctuations;

(d) converting said autocorrelation function to a two-component exponential decay curve;

(e) computing the amplitudes and fluctuation rates of said two components; and

(f) utilizing said amplitudes and fluctuation rates of said two components by relating them to two species of protein in said aqueous whereby the relative degree of laser light scattering by said two protein species in a patient'"'"'s aqueous can be normalized with respect to laser light scattered in a patient'"'"'s lens and correlated to a serum cholesterol level.