Apparatus for applying stress to cell cultures

• US 4,839,280 A
• Filed: 04/11/1988
• Issued: 06/13/1989
• Est. Priority Date: 05/04/1987
• Status: Expired due to Term

• Current Assignee: Flexcell International Corporation (Global PVQ SE)
• Original Assignee: Albert J. Banes
• Inventors: Banes, Albert J.
• Primary Examiner(s): Garrett, Robert E.
• Assistant Examiner(s): Price, Carl D.
• Application Number:
  US07/180,168
• Time in Patent Office: 428 Days
• Field of Search: 435/284, 435/285, 435/287, 435/300, 435/301, 435/310, 428/447, 428/116, 428/178, 524/17, 524/588
• US Class Current: 435/305.2
• CPC Class Codes: A61L 27/18 obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
• CPC Class Codes: C08J 2383/04 Polysiloxanes
• CPC Class Codes: C08J 7/14 with acids, their salts or anhydrides
• CPC Class Codes: C08L 83/04 Polysiloxanes
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 35/04 Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
• CPC Class Codes: C12N 2533/30 Synthetic polymers thermoreactive polymers, e.g. PNIPAm, C12N2539/10
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: G01N 2203/0023 Bending
• CPC Class Codes: G01N 2203/0046 Vacuum
• CPC Class Codes: G01N 2203/0089 Biorheological properties
• CPC Class Codes: G01N 33/54353 with ligand attached to the carrier via a chemical coupling agent coatings G01N33/54393
• CPC Class Codes: G01N 33/545 Synthetic resin
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates
• CPC Class Codes: Y10T 428/31663 As siloxane, silicone or silane

Abstract

A polyorganosiloxane composition having a biocompatible surface thereon is disclosed. The biocompatible surface results from the derivatization, or amination, of the surface intended for cell contact. More specifically, the present invention is a polyorganosiloxane composition in which the surface is either treated with a primary amine and optional peptide or the surface is cocured with a primary amine-containing silane or siloxane. The aminated polyorganosiloxane has utility as a cell culture substrate or in a variety of artificial organ applications such as breast implants, synthetic blood vessels, joints, tendons and heart valves. A vacuum apparatus for use with specialized cell culture plates incorporating the biocompatible polyorganosiloxane composition is also disclosed.

Claims

1. An apparatus for applying stress to cell cultures comprising:

at least one cell culture plate having one or more wells thereon, with each of said wells having a substantially planar base formed at least partially of an elastomeric membrane made of a biocompatible polyorganosiloxane composition, with said elastomeric membrane having an upper surface treated to permit cell growth and attachment thereto by means of the incorporation at said upper surface of a substance selected from the group consisting of an amine, a carboxylic acid, or elemental carbon, and vacuum means for controlling subjecting said elastomeric membrane to the pulling force of a vaccum.

2. The apparatus of claim 1 wherein said vacuum means includes a vacuum plenum connected by control means to a vacuum source and wherein said culture plates are carried by and are in contact with said vacuum plenum, with said vacuum plenum including means for communicating said vacuum to a bottom surface of said elastomeric membranes.

3. The apparatus of claim 2 wherein said vacuum plenum is a flat plate having at least one vacuum channel in an upper surface thereof, and further including a substantially flat gasket positioned adjacent the upper surface of said plenum and beneath said culture plates, with said gasket having one or more apertures therethrough which are aligned with and extend between an underlying vacuum channel and an elastomeric membrane of an overlying culture plate, wherein a sealed air chamber is formed which extends from the elastomeric membrane, and through the aperture and vacuum channel to said vacuum source.

4. The apparatus of claim 3 further including a flow means disposed between said plenum and said vacuum source and further comprising a control means for controlling the application of vacuum to said vacuum plenum.

5. The apparatus of claim 4 wherein said flow means is a solenoid valve and said control means is a computer electrically connected to said solenoid valve.