Device for the separation of the lighter fraction from the heavier fraction of a liquid sample

• US 4,843,017 A
• Filed: 10/27/1987
• Issued: 06/27/1989
• Est. Priority Date: 01/25/1985
• Status: Expired due to Fees

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Palmer, Phyllis J., Oberhardt, Bruce J.
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): KRYZA, LYNN M
• Application Number:
  US07/113,577
• Time in Patent Office: 609 Days
• Field of Search: 422/101, 422/104, 436/177, 436/178, 210/247, 210/321.6, 210/321.84, 210/433.1, 210/649, 128/760, 128/762, 128/763, 128/764, 604/414
• US Class Current: 436/177
• CPC Class Codes: B01D 61/18 Apparatus therefor
• CPC Class Codes: G01N 33/491 by separating the blood components G01N15/05 takes precedence
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]
• CPC Class Codes: Y10T 436/255 including use of a solid sorbent, semipermeable membrane, or liquid extraction

Abstract

A device for the separation of a lighter fraction from the heavier fraction of a liquid sample for use with two evacuated receptacles includes a housing having an interior cavity and a membrane separator dividing this cavity into a first portion and a second portion. The membrane separator has a porosity selected for the desired separation thereacross. An inlet structure is provided for fluid communication between the first portion of the interior cavity and the source of the liquid sample. First structure is provided to allow fluid communication between the first portion of the interior cavity and the first evacuated receptacle. This first structure is opposed from the inlet structure so that liquid passing from the inlet to the first structure travels in a direction along the surface of the membrane. Further structure is provided to allow fluid communication between the second portion of the interior cavity and the second evacuated receptacle.

Claims

1. A method of separating the lighter fraction from the heavier fraction of a liquid sample using a device including a housing having an interior cavity, a separator membrane dividing said cavity into a first portion and a second portion, said separator membrane having a porosity selected for the desired separation thereacross, inlet means for providing fluid communication between said first portion and the source of the liquid sample, first communication means for providing fluid communication between said first portion and a first evacuated receptacle, second communication means for providing fluid communication between said second portion and a second evacuated receptacle comprising:

establishing fluid communication between said inlet means and the source of the liquid sample; and

establishing fluid communication between a first evacuated receptacle and said first communication means and between a second evacuated receptacle and said second communication means so that the liquid sample flows through said inlet means along said membrane, through said first communication means and into said first evacuated receptacle, simultaneously, the lighter fraction of the liquid sample being drawn through said membrane and said second communication means into said second evacuated receptacle.

2. A method of separating plasma from a blood sample using a device including a housing having an interior cavity, a separator membrane dividing said cavity into a first blood receiving portion and a second plasma receiving portion, said separator membrane having a porosity selected for separating plasma from blood, inlet means for providing fluid communication between said blood receiving portion and the source of the blood sample, first communication means for providing fluid communication between said first portion and a first evacuated receptacle, second communication means for providing fluid communication between plasma receiving portion and a second evacuated receptacle comprising:

establishing fluid communication between said inlet means and the source of the blood sample; and

establishing fluid communication between a first evacuated receptacle and said first communication means and between a second evacuated receptacle and said second communication means so that the blood sample flows through said inlet means along said membrane, through said first communication means and into said first evacuated receptacle, simultaneously, plasma being drawn through said membrane and said second communication means into said second evacuated receptacle.

3. The method of claim 2 wherein the step of establishing fluid communication between said inlet means and the source of the blood sample includes inserting a needle cannula into a patient'"'"'s body so that blood is drawn directly from the patient into said inlet means.

4. A method of separating plasma from a blood sample using a first evacuated receptacle and a second evacuated receptacle comprising:

withdrawing blood from a patient;

directing said blood, as it is being withdrawn, along the surface of a separator membrane, having a porosity selected for separating plasma from blood, and into a first evacuated receptacle; and

simultaneously, causing plasma from said blood to be drawn through said membrane into said second evacuated receptacle.

5. A method of separating plasma from blood comprising:

withdrawing blood form a patient across the surface of a separator membrane, having a porosity selected for separating plasma from blood, and into a first evacuated receptacle; and

simultaneously drawing plasma from said blood through said membrane into a second evacuated receptacle while said blood is being withdrawn.