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Immunological assay method

  • US 4,843,021 A
  • Filed: 07/30/1987
  • Issued: 06/27/1989
  • Est. Priority Date: 07/30/1986
  • Status: Expired due to Fees
First Claim
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1. An immunological assay for an analyte in a sample wherein calibration curves for assay results are prepared based on a change in transmitted or scattered light caused by a change in turbidity accompanying formation of an antigen-antibody complex by an antigen-antibody reaction comprising:

  • providing a latex particle containing reagent having an average reagent particle diameter (D) in the range of 0.1 to 0.5 μ

    m, a D to d ratio (D/d), wherein d represents the average particle diameter of said latex particles, in the range of 1.3 to 3.0 and a zeta potential in the range of -20 to 10 mV, for use in said assay;

    obtaining calibration curves or parameters which make up calibration curves for said latex particle containing reagent used in the assay;

    providing said calibration curves or said parameters in a magnetic medium;

    outputting said calibration curves or calibration curves that have been prepared using of said parameters from said magnetic medium to an analyzer;

    said analyzer being incorporated into a computer;

    reacting in said analyzer said latex particle containing reagent with a sample;

    obtaining any change in transmitted or scattered light caused by any change inturbidity accompanying any formation of an antigen-antibody complex by an antigen-antibody reaction;

    and comparing any change in transmitted or scattered light with said calibration curves for said latex particle containing reagent in order to determine the amount of analyte in said sample.

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