Apparatus and method for testing liquids

• US 4,844,097 A
• Filed: 02/09/1987
• Issued: 07/04/1989
• Est. Priority Date: 05/21/1985
• Status: Expired due to Fees

• Current Assignee: Bellhouse Technology Limited 24 Blacklands Way Abingdon Oxford England
• Original Assignee: Bellhouse Technology Limited
• Inventors: Pugh, Sydney M., Bellhouse, Brian J.
• Primary Examiner(s): Richman, Barry S.
• Assistant Examiner(s): McMahon, Timothy M.
• Application Number:
  US07/006,653
• Time in Patent Office: 876 Days
• Field of Search: 422/68.01-68.07, 436/68, 436/150, 436/151, 436/175, 204/1 P, 204/415, 128/635, 128/637, 604/4
• US Class Current: 600/352
• CPC Class Codes: G01N 27/414 Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
• CPC Class Codes: G01N 33/49 Blood chemical methods for determining blood cell populations G01N33/5094; chemical analysis of blood groups or blood types G01N33/80

Abstract

The application relates to a method and apparatus for testing the concentration of a selected atomic scale species in a thick liquid, such as blood. The blood flows through a chamber that is in part defined by a microporous dialysis membrane permeable to atomic scale species. A measuring cell including a second chamber filled with a clean liquid is defined by the other plane of the membrane. The measuring cell incorporates a sensor having an electrode coated with a material permeable only to a selected ionic species.

Claims

1. Apparatus for testing the concentration of a selected atomic scale species in a thick liquid, the apparatus comprising a first chamber which contains, in use, a thick liquid under test, the chamber being at least partly defined by one face of a microporous dialysis membrane which is permeable to atomic scale species;

and a measuring cell incorporating a second chamber which is arranged to be filled with a clean liquid and which is at least partly defined by an opposite face of the membrane whereby atomic scale species in the thick liquid under test diffuse through the membrane and are present in a clean liquid in a concentration related to that at which they exist in a thick liquid under test;

the cell also incorporating a sensing device having an active surface which is responsive substantially only to a selected species and which comprises an electrod in communication with the clean liquid in the second chamber, said electrode being coated with a material which is permeable substantially only to a selected ionic species whereby the device provides an output related to the concentration of a selected species.

2. Apparatus according to claim 1 wherein the microporous membrane and the active surface of the sensor are spaced by substantially 0.1 mm.

3. Apparatus according to claim 1 wherein the second chamber is provided with inlet and outlet ducts for flushing the second chamber with clean liquid.

4. Apparatus according to claim 1 wherein the sensor is a dissolved gas sensing device having an electrode which is responsive to a selected gaseous species.

5. Apparatus according to claim 1 wherein the sensor is an ISFET having one or more gate electrodes coated with a material which is permeable substantially only to a selected ionic species.

6. Apparatus according to claim 1, for testing the concentration of dissolved gases in the thick liquid, wherein the microporous membrane is a hydrophobic membrane made of polypropylene or PTFE.

7. Apparatus according to claim 6, wherein the sensor is a dissolved gas sensing device having an electrode which is responsive to a selected gaseous species.

8. Apparatus according to claim 1 for testing the concentration of an ionic species, wherein the microporous membrane is a dialysis membrane.

9. Apparatus according to claim 8, wherein the sensor is an ISFET having one or more gate electrodes coated with a material which is permeable substantially only to the selected ionic species.

10. Apparatus according to claim 1, wherein the microporous membrane and the active surface of the sensor, which face one another, are spaced by a spacer formed with opening means which provide the second chamber.

11. Apparatus according to claim 10, wherein the spacer is a non-woven nylon or polyester mesh.

12. Apparatus according to claim 10 for testing the concentration of an ionic species, wherein the microporous membrane is a dialysis membrane.

13. Apparatus according to claim 1, wherein the first chamber is a blood line connector, having in its wall an opening means which is spanned by the microporous membrane, the cell being mounted on the outside of the connector overlying the membrane.

14. Apparatus according to claim 13 wherein the microporous membrane and the active surface of the sensor, which face one another, are spaced by a spacer formed with openings which provide the second chamber.

15. Apparatus according to claim 13 wherein the second chamber is provided with inlet and outlet ducts for flushing the second chamber with clean liquid.

16. Apparatus according to claim 15 wherein the microporous membrane and the active surface of the sensor which face one another, are spaced by a spacer formed with opening means which provide the second chamber.

17. Apparatus according to claim 15, wherein the inlet and outlet ducts are closeable to seal the interior of the cell during test.

18. Apparatus according to claim 17 wherein the microporous membrane and the active surface of the sensor, which face one another, are spaced by a spacer formed with opening means which provide the second chamber.

19. A method of testing the concentration of a selected atomic scale species in a flow of natural whole blood in an extracorporeal circuit, the method comprising continuously circulating the blood through a conduit having in its wall an aperture spanned by a microporous barrier membrane which is permeable to atomic scale species, but impermeable to proteins and other blood components of similar and larger size, whereby unselected atomic scale species pass through the barrier membrane into a measuring cell containing a clean liquid;

sensing the concentration of the selected species in the clean liquid by means of a sensing device, which is responsive substantially only to the selected species, said device comprising an electrode coated with a material which is permeable substantially only to a selected ionic species; and

providing an output related to the concentration of the selected species.

20. A method according to claim 19, wherein the sensor comprises an electrode coated with a material, which is permeable substantially only to a selected ionic species under test, but which is foulable by blood components retained by the barrier membrane.