Method of displaying multi-parameter data sets to aid in the analysis of data characteristics

• US 4,845,653 A
• Filed: 05/07/1987
• Issued: 07/04/1989
• Est. Priority Date: 05/07/1987
• Status: Expired due to Term

• Current Assignee: Becton, Dickinson & Co
• Original Assignee: Becton, Dickinson & Co
• Inventors: Bezdek, James C., Conrad, Morgan P., Reichert, Thomas A.
• Primary Examiner(s): Harkcom, Gary V.
• Assistant Examiner(s): Zimmerman, Mark K.
• Application Number:
  US07/046,619
• Time in Patent Office: 789 Days
• Field of Search: 364/172, 364/194, 364/415, 364/497, 364/521, 364/554, 364/555, 364/413.1, 364/510, 364/413.08, 364/526, 340/747
• US Class Current: 715/805
• CPC Class Codes: G01N 2015/1488 Methods for deciding
• CPC Class Codes: G06F 3/04842 Selection of displayed objects or displayed text elements G06F3/0482 takes precedence
• CPC Class Codes: Y10S 715/97 Instrumentation and component modelling, e.g. interactive control panel

Abstract

The method involves displaying at least three different parameters of particle-related data in multiple two-parameter data fields on a screen for viewing by a user. A position variable cursor is applied to any two-parameter data field and a region of data events is created within the data field by the cursor. Data events in the created region are linked and corresponding data events, defined by all other parameter data fields displayed on the screen, are then located. This method includes visually differentiating the data events in the other data fields which correspond to the data events in the created region of the first data field. This differentiation permits the user to identify these same data events on all of the two-parameter data fields to thereby facilitate visual exploratory data analysis.

Claims

1. A method for analyzing multi-parameter, cell related data comprising:

displaying a plurality of data fields on a screen for viewing by a user, each data field defined by two axes representing two parameters relating to characteristic of cells, at least one parameter of one of said data fields being different from the parameters of the remaining data fields so that at least three different parameters of cells are represented in the plurality of data fields;

applying a position variable cursor to one of said data fields and creating a region of data events within said one data field by use of said cursor;

linking the data events in said region with corresponding data events defined by different parameters in the remaining data fields on the screen; and

visually differentiating the displayed data events in the remaining data fields corresponding to the events in said created region of said one data field so that the user may identify the linked data events on all of said two-parameter data fields which correspond with each other.

2. The method of claim 1 wherein said displaying step includes displaying data fields in which four different parameters of cells are represented in the plurality of data fields.

3. The method of claim 2 wherein said four different parameters are displayed in six different two-parameter data fields on said screen.

4. The method of claim 1 wherein said displaying step includes displaying data fields simultaneously on a plurality of screens.

5. The method of claim 1 wherein said displaying step includes displaying data fields representing different parameters obtained in conjunction with cells passing through a flow cytometry instrument.

6. The method of claim 1 wherein said data fields are dot plots.

7. The method of claim 1 wherein at least one of said data fields is a histogram.

8. The method of claim 1 wherein said creating step includes using said cursor to trace a region and form an enclosure of data events.

9. The method of claim 1 wherein said creating step includes using said cursor as a brush to paint over data events on said one data field so that all such painted events are included in the region.

10. The method of claim 1 wherein said creating step includes using said cursor as a variable size box in which all data events falling within the boundaries of the box are in the region.

11. The method of claim 1 wherein said applying step includes moving the cursor on the data field by manual manipulation of a mouse which is electrically connected to said cursor and wherein the cursor is position- sensitive depending upon the position of the mouse.

12. The method of claim 1 wherein said linking step includes electrically accessing all the data events in said region and directing a signal to each remaining data field to identify each such data event defined by the two different parameters in each such remaining data field.

13. The method of claim 12 wherein said accessing step includes using a linked listing of multi-parameter data stored in memory so that each data event found in the region of said one data field may be correspondingly located in the remaining data fields but identified by the different parameters thereof.

14. The method of claim 1 wherein the differentiating step includes causing the data events in the region of said one data field and the corresponding data events in the remaining data fields to have a brightness on the screen different from data events not included within said region.

15. The method of claim 1 wherein the differentiating step includes causing the data events in the region of said one data field and the corresponding data events in the remaining data fields to intermittently blink.

16. The method of claim 1 wherein the differentiating step includes causing the data events in the region of said one data field and the corresponding data events in the remaining data fields to have a color different from data events not included in said region.

17. The method of claim 16 wherein the step of differentiating the data events includes using the cursor to adjust the size of the colored region to a larger or smaller state after the original color region has been created.

18. The method of claim 1 which further includes creating a plurality of regions of data events on said one data field and linking the data events in each respective region with corresponding data events in the remaining data fields on the screen.

19. The method of claim 18 wherein the differentiating step includes causing the data events in each region of said one data field and the corresponding data events in the remaining data fields to have a color different from each other and from data events not included in any of the regions.

20. The method of claim 19 wherein the differentiating step includes causing the colors of the different regions to overlap each other.

21. The method of claim 19 wherein the step of differentiating the data events includes eliminating all or portions of the color of any region after the original colored region has been created.

22. The method of claim 1 wherein the step of creating a region of data events on said one data field further includes using the cursor to adjust the size of the region to a larger or smaller state after the original region has been created.

23. The method of claim 1 wherein said applying step includes selectively and interchangeably applying the cursor to any other of said data fields after said cursor has been applied to said one of the data fields.

24. The method of claim 1 wherein the differentiating step includes distinguishing different types of cells.

25. The method of claim 24 wherein the differentiating step includes distinguishing different subpopulations of a type of cells.

26. The method of claim 1 further including storing coordinates of the boundaries of said region for subsequent access.

27. A method for analyzing multi-parameter data obtained by flow cytometric procedures comprising:

displaying particle-related data in a plurality of separate, two-parameter data fields on a screen for viewing by a user;

applying a position variable cursor to any two-parameter data field and creating a region within said data field by use of said cursor;

linking said region to all data events within said region;

locating data events on all other two-parameter data fields on the screen which correspond to the linked data events; and

visually differentiating all linked and corresponding events so that the user may readily identify all such data events.

28. A method for analyzing multi-parameter data comprising:

displaying data in a plurality of separate, two-parameter data fields on a screen for viewing by a user;

applying a position variable cursor to any two-parameter data field and creating a region within said data field by use of said cursor;

linking said region to all data events within said region;

locating data events on all other two-parameter data fields being displayed which correspond to the linked data events; and

visually differentiating all linked and corresponding events so that the user may readily identify all such data events.

29. The method of claim 28 wherein said displaying step includes displaying data including events which have been obtained from multiple sources.

30. A method for analyzing data to determine one or more characteristics of cells comprising:

displaying a plurality of two-parameter data fields on a screen for viewing by a user, said fields representing different displays of different parameters relating to characteristics of cells;

applying a position-variable cursor to one of said data fields and moving said cursor to enclose selective data events in a defined region;

linking the data events in said region and located corresponding events defined by different parameters in the remaining data fields on the screen by accessing a linked listing of multi-parameter data related to said cells stored in memory so that each data event falling in said region is correspondingly located in the remaining data fields, but identified by the different parameters thereof; and

visually differentiating the data events in the remaining data fields corresponding to the events in said defined region by causing all the differentiated data events to have a color different from data events not included in said region so that the user may identify the data events on all of said two-parameter data fields which correspond with each other for purposes of cellular analysis.