Detection and indentification of bacteria by means of ultra-violet excited resonance Raman spectra

• US 4,847,198 A
• Filed: 10/07/1987
• Issued: 07/11/1989
• Est. Priority Date: 10/07/1987
• Status: Expired due to Term

• Current Assignee: Rhode Island Board of education (STATE OF RHODE ISLAND)
• Original Assignee: Rhode Island Board of education (STATE OF RHODE ISLAND)
• Inventors: Sperry, Jay F., Nelson, Wilfred H., Dalterio, Richard A.
• Primary Examiner(s): NOT, DEFINED
• Assistant Examiner(s): Wagner, Richard W.
• Application Number:
  US06/916,214
• Time in Patent Office: 643 Days
• Field of Search: 356/301, 435/29, 435/34, 435/173, 435/808, 436/63
• US Class Current: 435/34
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence
• CPC Class Codes: Y10S 435/808 Optical sensing apparatus

Abstract

A bacterial suspension is contacted by a single vavelength in the ultra-violet range. A portion of the light energy used is absorbed and a portion of the light energy is emitted. The emitted light energy, resonance enhanced Raman scattering, is measured as backscattered energy. The energy is processed to produce spectra which are characteristic of the bacteria.

Claims

1. A method for the identification of a bacterium which comprises:

exciting taxonomic markers in a bacterium with a beam of uItra violet energy, some of said energy emitted from the bacterium as a lower resonance enhanced Raman back scattered energy;

collecting the resonance enhance Raman back scattered energy substantially in the absence of fluoresence;

converting the resonance enhanced Raman back scattered energy into spectra which corresponds to the taxonomic markers in said bacterium; and

displaying the spectra whereby the bacterium may be identified.

2. The method of claim 1 wherein the beam of energy is in the wavelength range of 190 to 270 nm.

3. The method of claim 1 wherein the back scattered energy is collected at an angle of between 0°

to 90°

based on the angle between the beam of energy and the axis along which the back scattered energy is collected.

4. The method of claim 1 wherein the displaying of the spectra includes:

plotting the spectral lines.

5. The method of claim 1 which includes:

plotting the spectral lines as a function of wavelength.