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Latex agglutination immunoassay in the presence of hemoglobin

  • US 4,847,209 A
  • Filed: 11/09/1987
  • Issued: 07/11/1989
  • Est. Priority Date: 11/09/1987
  • Status: Expired due to Term
First Claim
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1. In an analytical method for determining an analyte in a lysed blood sample by latex agglutination immunoassay, wherein (a) an aqueous reaction mixture is formed by combining the lysed blood sample with a multivalent latex antibody reagent comprising an anti-analyte antibody, or a fragment thereof, bound to a water suspensible latex particle, and additionally when the analyte is monovalent, with an agglutinator reagent comprising a plurality of epitopic binding sites for the anti-analyte antibody or fragment thereof, and (b) the resulting agglutination in the aqueous reaction mixture is measured as a function of analyte in the blood sample,the improvement which comprises forming the aqueous reaction mixture to have a pH of about 8.5 or greater, whereby nonspecific agglutination of the latex reagent due to the presence of hemoglobin in the blood sample is substantially overcome.

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