Oligonucleotide derivatives and production thereof

• US 4,849,336 A
• Filed: 04/25/1986
• Issued: 07/18/1989
• Est. Priority Date: 02/14/1983
• Status: Expired due to Term

• Current Assignee: Wakunaga Seiyaku Kabushiki Kaisha
• Original Assignee: Wakunaga Seiyaku Kabushiki Kaisha
• Inventors: Suzuki, Masanori, Miyoshi, Kenichi, Fuwa, Toru
• Primary Examiner(s): Kepplinger, Esther M.
• Application Number:
  US06/855,710
• Time in Patent Office: 1,180 Days
• Field of Search: 435/6, 435/810, 935/78, 436/501
• US Class Current: 435/6.12
• CPC Class Codes: C07H 19/16 Purine radicals
• CPC Class Codes: C07H 19/20 with the saccharide radical esterified by phosphoric or polyphosphoric acids
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: G01N 33/535 with enzyme label or co-enzymes, co-factors, enzyme inhibitors or enzyme substrates
• CPC Class Codes: Y10S 435/81 Packaged device or kit

Abstract

Biotin- or 2,4-dinitrophenyl-oligodeoxyribonucleotide derivative represented by the formula shown below and its preparation method is disclosed: ##STR1## wherein: R is ##STR2## m and n are each 0 or a natural number; R¹ is a straight or branched hydrocarbon residue; and B is a base constituting the nucleotide (in which when a plural number of B exist, they may be either identical or different). This compound is useful for use as a nonradioactive affinity probe for nucleic acid or a primer.

Claims

1. A kit for the non-radioactive detection of DNA or RNA, comprising:

(i) a nucleotide comprising an oligonucleotide of the formula;

##STR16## wherein R is a biotin moiety or a 2,4-dinitrobenzene moiety having, respectively, of the following formulae;

##STR17## (m+n) is an integer from 1 to 46 R¹ is a straight or branched C₂ -C₂₀ alkylene; and

each B may be the same or different and is at least one nucleotide residue carrying a base B selected from the group consisting of adenine, cytosine, guanine and thymine; and

(ii) a visualizing aid.

2. The non-radioactive hybridization probe of claim 1 wherein m is 1-4 and n is 0-20.

3. The non-radioactive hybridization probe of claim 1 wherein R is ##STR18##

4. The non-radioactive hybridization probe of claim 1 wherein R is ##STR19##