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Apparatus for mechanically stimulating cells

  • US 4,851,354 A
  • Filed: 12/07/1987
  • Issued: 07/25/1989
  • Est. Priority Date: 12/07/1987
  • Status: Expired due to Fees
First Claim
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1. A method for studying cells in culture under conditions which reproduce their natural, in vivo mechanical environment comprisingproviding a device comprisingan airtight well having an optically transparent circular compliant base of a biologically compatible material on which said cells may be grown and an optically transparent, removable cap, which airtight well and removable cap are formed of biologically compatible materials, anda ported, airtight reservoir coupled with said well beneath said compliant base, which reservoir has an optically transparent base;

  • seeding said cells on said compliant base;

    allowing said cells to become confluent and attach to said compliant base;

    injecting into and withdrawing from said airtight reservoir gaseous medium to create cyclic variations in hydrostatic pressure beneath said compliant base to concavedly-convexedly deform and thereby exert a substantially uniform biaxial strain on the cells attached thereto.

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