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Liver assist device employing transformed cell lines

  • US 4,853,324 A
  • Filed: 11/17/1986
  • Issued: 08/01/1989
  • Est. Priority Date: 12/02/1985
  • Status: Expired due to Term
First Claim
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1. A method of preparing a semipermeable membrane presenting a pair of opposed faces for use as a liver assist device, said method comprising the steps of:

  • providing a mass of priamry hepatocytes;

    reversibly transforming said hepatocytes to cause the hepatocytes to proliferate in vitro, and to decrease the contact inhibition thereof;

    culturing said reversibly transformed hepatocytes on one face of said membrane until at least a confluent monolayer of reversibly transformed hepatocytes is formed thereon,said culturing step comprising the steps of seeding said one face with an effective quantity of said reversibly transformed hepatocytes to form said confluent monolayer, and continuously circulating culture medium which supports the growth of said reversibly transformed hepatocytes into contact with the reversibly transformed hepatocytes for a period of from about 1 to 48 days; and

    causing said cultured, reversibly transformed hepatocytes to revert to the somatic phenotype thereof.

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