Device for analyzing a liquid sample

• US 4,857,274 A
• Filed: 06/22/1987
• Issued: 08/15/1989
• Est. Priority Date: 06/26/1986
• Status: Expired due to Fees

• Current Assignee: KIS Photo Industrie A French Incorporation
• Original Assignee: KIS Photo Industrie
• Inventors: Simon, Jean-Pierre
• Primary Examiner(s): Marcus, Michael S.
• Application Number:
  US07/064,696
• Time in Patent Office: 785 Days
• Field of Search: 422/61, 422/72, 422/102, 436/165, 436/177, 436/180, 356/426, 356/427, 250/576
• US Class Current: 422/72
• CPC Class Codes: G01N 21/07 Centrifugal type cuvettes G01N21/09 takes precedence

Abstract

A device for analyzing a liquid sample such as blood includes a cartridge portion and a casing portion. The cartridge includes an inlet for directly receiving a sample. The cartridge is then irreversibly clipped to the casing so that an inlet of the casing is in communication with an outlet of the cartridge. The sample is then mixed in the casing with a diluent and/or reagent by using a centrifuge, and measurements are taken. The device allows a sample to be taken directly into the cartridge, and restricts employment of the device to a single use.

Claims

1. A device for analyzing a sample of a liquid comprising:

a compartmented casing comprising an inlet, a container for at least one dose of a reagent of diluent, a chamber for calibration of a calibrated quantity obtained from the sample, and a chamber for mixing at least the calibrated quantity and the reagent of diluent, such that when said casing interacts with a centrifuging apparatus centrifugal force causes contents of the casing to circulate within the casing; and

a cartridge including an inlet for directly introducing a sample into the cartridge from a source, a receptacle for collecting the introduced sample, means for metering the collected sample, said means for metering connected to the receptacle via a capillary duct extending into the casing, means for irreversibly clipping the cartridge to said casing, and an outlet passage communicating with the casing inlet when said cartridge is irreversibly clipped to said casing to pass a metered quantity from said cartridge to said casing, wherein said outlet of the cartridge is positioned in a vicinity of, but separate from, said cartridge inlet.

2. A device as claimed in claim 1, wherein the metering means incorporates means for separating the sample to be analyzed, by centrifuging, into at least two fractions, one light and one heavy, said means for separating permitting the light fraction of the sample to be drained toward the outlet of the cartridge, and an overflow cell connected to the metering means.

3. A device as claimed in claim 2, wherein the separating means comprises at least two distinct chambers interconnected via a constricted passage, and one of the two distinct chambers serves to collect the heavy fraction.

4. A device as claimed in claim 3, wherein the separating means comprises three chambers interconnected via at least two capillary passages, and two of the three chambers serve to collect, successively, the heavy fraction of the sample.

5. A device as claimed in claim 1, wherein the cartridge is bileveled and comprises a level on which the liquids circulate under the action of the centrifugal force, and a level which is at least partly allocated to the overflow cell.

6. A device as claimed in claim 1, wherein the calibration chamber is a metering siphon comprising two capillary ducts, one for introducing the metered quantity originating from the cartridge, and the other for draining the overflow, connected to an overflow chamber.

7. A device as claimed in claim 1, wherein the container for the reagent is contained within the casing, and can be displaced by pressure applied externally to said casing, from a first position in which the container is closed in a leaktight manner, to a second definitive activation position, in which a capillary aperture is opened in the container, toward the mixing chamber.