Carbon and energy source markers for transformation of strains of the genes Pichia

• US 4,857,467 A
• Filed: 07/23/1986
• Issued: 08/15/1989
• Est. Priority Date: 07/23/1986
• Status: Expired due to Term

• Current Assignee: Research Corporation Technologies
• Original Assignee: Phillips Petroleum Company (ConocoPhillips)
• Inventors: Fuke, Motohiro, Sreekrishna, Kotikanyadan
• Primary Examiner(s): Weimar, Elizabeth C.
• Assistant Examiner(s): Carson, P.
• Application Number:
  US06/887,300
• Time in Patent Office: 1,119 Days
• Field of Search: 435/254, 435/255, 435/938, 435/911, 435/921, 435/940, 435/944, 435/34, 935/69, 935/66, 935/97
• US Class Current: 435/254.23
• CPC Class Codes: C12N 15/65 using markers enzymes used as markers C12N15/52
• CPC Class Codes: C12N 15/81 for yeasts
• CPC Class Codes: C12N 15/815 for yeasts other than Saccharomyces
• CPC Class Codes: C12N 9/2408 acting on alpha -1,4-glucosidic bonds
• CPC Class Codes: Y10S 435/938 Pichia

Abstract

Yeast strains are transformed with, and selected for, using DNA fragments encoding a gene function not present in the wild-type yeast strains. The invention requires transforming the yeast with DNA fragments which comprise the gene function which the wild-type yeast strain is lacking; regeneration of the transformants on a non-selective regeneration medium; then selection of those colonies which are capable of growth on a carbon and energy source which requires expression of the gene function provided by the DNA fragment. Thus, transformation with such DNA fragments allows for positive selection of transformed strains.

Claims

1. The pure culture comprising Pichia pastoris NRRL y-11430/pTSUI.

2. The pure culture comprising Pichia pastoris y-11430/pSR11.

3. The pure culture comprising Pichia pastoris NRRL y-15851/pSR1.