Light emitting diode spectrophotometer

• US 4,857,735 A
• Filed: 10/23/1987
• Issued: 08/15/1989
• Est. Priority Date: 10/23/1987
• Status: Expired due to Fees

• Current Assignee: Trenton Laboratories Co.
• Original Assignee: Hans G. Noller
• Inventors: Noller, Hans G.
• Primary Examiner(s): Howell, Janice A.
• Assistant Examiner(s): NOT, DEFINED
• Application Number:
  US07/113,230
• Time in Patent Office: 662 Days
• Field of Search: 250/339, 250/343, 250/345, 250/341, 356/323, 356/319, 356/435, 356/217
• US Class Current: 250/339.07
• CPC Class Codes: G01N 2021/3181 using LEDs
• CPC Class Codes: G01N 21/3151 using two sources of radiation of different wavelengths G01N21/33 - G01N21/39 take precedence

Abstract

A spectrophotometer incorporating a pulsed light emitting diode as a source of radiation. The light emitting diode emits substantially monochromatic light thus negating the need for a separate wavelength control. The spectrophotometer may incorporate a pair of light emitting diodes for performing bichromatic spectrophotometric determinations. The light emitting diodes are pulsed with a duty cycle and pulse amplitude such that it is possible to obtain a higher amplitude pulse than the light emitting diode could sustain at a continuous voltage input level. Continuous voltages at this level would damage the light emitting diode.

Claims

1. A spectrophotometer with a high signal to noise ratio, comprising:

a first light emitting diode for emitting light through a solution having a spectrophotometric absorbance maximum within the band of wavelengths of light emitted by said light emitting diode;

a first pulse generator connected to said light emitting diode, said pulse generator providing pulses having a duty cycle and voltage selected so that said light emitting diode emits light of higher intensity during pulses generated by said pulse generator than that maximum intensity that said light emitting diode could produce with a continuous voltage without damage to said light emitting diode;

a photodetector optically coupled to said light emitting diode for detecting light emitted from said light emitting diode and passed through said solution; and

a first signal generator coupled to said photodetector for generating a signal representative of the intensity of the light detected by said photodetector.

2. A spectrophotometer as claimed in claim 1 wherein said light emitting diode emits radiation with a wavelength in the infrared region of the light spectrum.

3. A spectrophotometer as claimed in claim 1 wherein said light emitting diode emits light in the visible portion of the light spectrum.

4. A spectrophotometer as claimed in claim 1 wherein said light emitting diode emits light with a wavelength of approximately 570 nanometers.

5. A spectrophotometer as claimed in claim 1, additionally comprising a first signal converter coupled to said signal generator for providing a second signal representative of the absorbance of the solution of the light emitted by said light emitting diode.

6. A spectrophotometer as claimed in claim 5 additionally comprising a second signal converter coupled to said first signal converter, for providing a third signal representative of the percent absorbance of the solution of the light emitted by said light emitting diode.

7. A spectrophotometer as claimed in claim 6 wherein said second signal converter is coupled to said first signal converter.

8. A spectrophotometer as claimed in claim 7 wherein said first signal converter comprises an operational amplifier.

9. A spectrophotometer as claimed in claim 8 wherein said first signal converter comprises a logarithmic amplifier.

10. A spectrophotometer as claimed in claim 9 wherein said logarithmic amplifier is temperature compensated.

11. A spectrophotometer as claimed in claim 10 wherein said second signal converter comprises an operational amplifier.

12. A spectrophotometer as claimed in claim 1 wherein said pulse generator provides a pulse stream having a duty cycle of approximately 0.1 to 1 percent, and an amplitude of approximately 4 volts.

13. A spectrophotometer as claimed in claim 12 wherein said spectrophotometer includes an indicator coupled to said first signal generator for providing an indication representative of the intensity of the detected light, said indicator includes an array of light emitting diodes, said array constructed to display a numeral representing the amount of light detected by said photodetector.

14. A spectrophotometer as claimed in claim 1 additionally comprising:

a second light emitting diode for emitting light through a second solution having a spectrophotometric absorbance maximum within the band of wavelengths of light emitted by said second light emitting diode;

a second pulse generator connected to said second light emitting diode, said pulse generator providing pulses having a duty cycle and voltage selected so that said light emitting diode emits light of higher intensity during pulses generated by said pulse generator than that maximum intensity that said light emitting diode could produce with a continuous voltage without damage to said light emitting diode;

a second photodetector optically coupled to said second light emitting diode for detecting light emitted from said second light emitting diode and passed through said second solution;

a second signal generator coupled to said second photodetector for generating a signal representative of the intensity of the light detected by said second photodetector;

a third signal generator for generating a third signal representative of the difference in the intensities of the light detected by said photodetectors; and

an indicator coupled to said first and second generators through said third generator in order to provide an indication representative of the intensity of the detected light.

15. A spectrophotometer as claimed in claim 14 wherein said second signal generator comprises an operational amplifier and said third signal generator comprises a difference amplifier.

16. A high signal to noise spectrophotometer comprising:

a laser diode for emitting radiation through a solution having a spectrophotometric absorbance maximum within the band of wavelengths emitted by said laser diode;

a pluse generator connected to said laser diode said pulse generator providing pulses having a duty cycle and voltage selected, so that said laser diode emits higher intensity radiation during pulses generated by said pulse generator then that maximum intensity that said laser diode could produce with a continuous voltage without damage to said laser diode;

a photodetector, positioned so it that detects the main portion of the direct beam of radiation emitted by said laser diode, the main portion being partially absorbed when passed through said solution; and

a first signal generator coupled to said photodetector for generating a signal representative of the intensity of the radiation detected by said detector.

17. A method of optically measuring properties of a solution comprising the steps of:

(a) pulsing a light emitting diode with pulses having a duty cycle and voltage selected so that said light emitting diode emits light of higher intensity during pulses generated by said pulse generator than that maximum intensity that the light emitting diode could produce with a continuous voltage;

(b) passing light from the light emitting diode through a first solution;

(c) detecting the light after it passes through said first solution;

(d) generating a first signal representative of the intensity of the light detected in said detecting step; and

(e) generating from said first signal an indication relating to the intensity of the light detected.

18. A method as claimed in claim 17, comprising an additional step:

(f) performing each of said steps (a) through (d) for each solution in a group of solutions, said solutions in said group having spectrophotometric absorbance maxima within the band of wavelengths of light emitted by said light emitting diode, said group of solutions comprising;

a first solution containing ferricyanide and KCN;

a second solution containing dimethylaminobenzoic acid, 3-dimethylaminobenzoic acid, glucose oxidase, and peroxidase; and

a third solution containing diazotized sulfanilic acid.

19. A method as claimed in claim 18, wherein said group additionally comprises:

a fourth solution including bromcresol green solution;

a fifth solution containing phenolphthalein monophosphate solution with MgCl₂ ; and

a sixth solution containing orthocresolphthalein complexone and buffered diethylamine.

20. A method as claimed in claim 18 wherein said light emitting diode emits light with a wavelength of approximately 570 nanometers.

21. A method as claimed in claim 17 wherein said light emitting diode in said step (a) emits radiation with a wavelength in the infrared region of the light spectrum.

22. A method as claimed in claim 17 wherein said light emitting diode emits light with a wavelength of approximately 490 nanometers.

23. A method as claimed in claim 17 wherein said light emitting diode emits light with a wavelength between approximately 580 and 600 nanometers.

24. A method as claimed in claim 17 wherein said light emitting diode emits light with a wavelength between approximately 630 and 670 nanometers.

25. A method as claimed in claim 17 additionally comprising the steps of:

(f) pulsing a second light emitting diode with pulses having a duty cycle and voltage selected so that said second light emitting diode emits light of higher intensity during pulses generated by said pulse generator than that maximum intensity that the light emitting diode could produce with a continuous voltage without damage to the second light emitting diode;

(g) passing light from the second light emitting diode through a second solution;

(h) detecting the light emitted from said second light emitting diode after it passes through said second solution;

(i) generating a second signal representative of the intensity of the light detected in said step (h); and

(j) generating a third signal which is representative of the difference in the intensities of the light detected in said steps (c) and (h),

said indication being generated directly from said third signal and indirectly from said first and second signals.