Selectively ion-permeable dry electrodes for analyzing selected ions in aqueous solution

• US 4,859,306 A
• Filed: 05/31/1988
• Issued: 08/22/1989
• Est. Priority Date: 12/23/1985
• Status: Expired due to Fees

• Current Assignee: Iqbal W. Siddiqi Phd
• Original Assignee: Hoffmann-La Roche Incorporated (Roche Holding AG)
• Inventors: Wuhrmann, Hans-Rudolf, Siddiqi, Iqbal
• Primary Examiner(s): TUNG, TA HSUNG
• Application Number:
  US07/203,550
• Time in Patent Office: 448 Days
• Field of Search: 204/56.1, 204/91, 204/416-419, 204/435
• US Class Current: 204/416
• CPC Class Codes: G01N 27/3335 the membrane containing at least one organic component G01N27/3271 takes precedence; aspects concerning the enzyme reagent in enzyme electrodes C12Q1/001

Abstract

The electrode comprises an internal reference half-cell at stable potential comprising a conductive sheet, e.g. of metal of carbon, covered by a redox system. The redox system comprises a thin dehydrated layer of Prussian blue deposited by contact between the sheet and a mixture of alkaline ferrocyanide and a ferric salt.

Claims

1. A multilaminar dry electrode for determining the concentration of a specific ion in an aqueous solution, such as biological fluid, comprising:

(a) an electrochemical reference cell at stable potential having a conductive base layer and a layer of Prussian blue adhered firmly to the conductive base layer; and

(b) an ion selective diaphragm which is selected for the specific ion to be detected said ion selective diaphragm being deposited as a film on at least a Portion of the layer of Prussian blue;

whereby, when a drop of aqueous solution containing the specific ion to be detected is placed on the ion selective diaphragm and an external electrode is electrically interconnected with the diaphragm by the aqueous solution a concentration of the ion to be detected in the drop of aqueous solution may be determined by measuring an electrical potential between the electrochemical reference cell and the external electrode without the multilaminar dry electrode requiring any pre-treatment.

2. A multilaminar dry electrode according to claim 1, wherein the thickness of the Prussian blue layer is more than 0.01 μ

.

3. A multilaminar dry electrode according to claim 1, further comprising means for stabilizing the Potential drift of said multilaminar dry electrode such that the Potential drift after two minutes of stabilization does not exceed ±

0.2 mV-min.

4. A multilaminar dry electrode according to claim 1, wherein the Prussian blue layer is dry, and has a water content not exceeding 0.1%.