Composite substrate, intended for an apparatus for quantitative detection of a component present in a gas or liquid

• US 4,860,573 A
• Filed: 07/17/1987
• Issued: 08/29/1989
• Est. Priority Date: 10/03/1985
• Status: Expired due to Fees

• Current Assignee: Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Juliana
• Original Assignee: Nederlandes Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno
• Inventors: Barendsz, Nieuwenhuizen, Maarten S.
• Primary Examiner(s): Levy, Stewart J.
• Assistant Examiner(s): Roskos, Joseph W.
• Application Number:
  US07/061,281
• Time in Patent Office: 774 Days
• Field of Search: 73/23
• US Class Current: 73/19.1
• CPC Class Codes: G01N 2291/0256 Adsorption, desorption, surface mass change, e.g. on biosensors
• CPC Class Codes: G01N 2291/0423 Surface waves, e.g. Rayleigh waves, Love waves
• CPC Class Codes: G01N 29/022 Fluid sensors based on microsensors, e.g. quartz crystal-microbalance [QCM], surface acoustic wave [SAW] devices, tuning forks, cantilevers, flexural plate wave [FPW] devices microdevices per se B81B

Abstract

A substrate for an apparatus for quantitative detection of a component present in a gas or liquid, in which substrate elastic vibrations can be generated, said substrate being provided with a surface layer adapted to bond said component, so that the vibration behavior of said layer is changed. At least the surface portion of said substrate consists of a substance with active sites (A) for covalent bonding situated at its free surface, to which sites (A) molecules of a substance (X) are covalently bonded which, on the other hand, possess active sites (B) for selectively and reversibly bonding by means of a coordinative bond of the molecules of the component (G) to be detected. In particular between each active site (A) of the substrate and a molecule of the component bonding substance (X) a molecule of a coupler (K) can be inserted, which is covalently bonded to an active site (A) of said substrate, and, on the other hand, is bonded by means of a second bond (C) with a molecule of the component bonding substance (X), said second bond being a substantially covalent bond or a substantially coordinative bond. The component bonding substance (X) can be a substance in the form of an inclusion compound (X₁), a complexing compound (X₂), a metal ion cluster (X₃), or an enzyme (X₄). The coupler (K) can be formed by a trialkoxysilane compound with general formula (RO)₃ Si(CH₂)_n -Y, in which Y is adapted to provide the covalent or coordinative bond (C) with the component bonding substance (X), and comprises an active group, which coupler (K) can be a compound of the following kind (Formula I).

Claims

1. A composite substrate for use in an apparatus for the quantitative detection of a substance present in a gas or liquid of the type having transducer means for inducing elastic vibrations in a surface layer of the substrate and detector means for sensing changes in the elastic vibrations resulting from bonding of the substance to be detected with the surface layer, the improvement wherein the surface layer (D'"'"') of said substrate (D) comprises a first substance having first active sites (A) adapted for covalent chemical bonding and molecules (X) of a second substance, said molecules (X) having at least one first active site forming a covalent bond with a respective active site (A) of said substrate (D'"'"'), and having at least one second active site (B) different from said first site, said second active sites (B) of said molecules (X) being adapted for selectively and reversibly making a coordinative bond with molecules (G) of the substance to be detected.

2. The substrate of claim 1, characterised in that said second substance (X) is an inclusion compound (X₁), activatable or non-activatable by metal ions, such as cyclodextrine or a crown ether;

a complexing compound (X₂);

a metal ion cluster (X₃);

or an enzyme (X₄).

3. The substrate of claim 1, characterised in that the complexing compounds forming substance (X) is chosen on:

(a) phthalocyanine compounds comprising H, Si or a metal ion;

(b) porphryine compounds, with or without H, Si or a metal ion;

(c) ferrocene compouns or this kind with an other metal; and

(d) diketones or aminopolycarboxylates, comprising a metal ion such as a lanthanide ion.

7. The substrate of claim 1 or claim 4, characterised in that said substance (X) comprises one or more molecules each with a plurality of active sites (B).

8. The substrate of claim 1 or 4, characterized in that said surface layer comprises a material selected from the group of silicon dioxide (SiO₂) or a piezo-electric oxide such as zinc oxide (ZnO), and in that the active sites (A) of said surface layer are formed by OH-groups.

9. The substrate of claim 1, characterised in that the coupling molecule (K) is a trialkoxysilane compound with the general formula:

space="preserve" listing-type="equation">(RO).sub.3 Si(CH.sub.2).sub.n --Y

in which Y is adapted to provide the covalent or coordinative bond (C) with said second substance (X), and R is an alkyl group with between 1 and 6 C-atoms and n is a number between 1 and 6, Y comprising an active group selected from the group comprised of a carboxylate, an amino compound, a pyridine derivative, an imidazole, an aldehyde, a halogen, a diazo compound, an acid halogenide, an axide, a sulphonate, a thioketone or hydroxyl compound.

10. The substrate of claim 1, characterised in that said coupling molecule (K) is a compound selected from the group characterized by the formula:

##STR2##

4. A composite substrate for use in an apparatus for the quantitative detection of a substance present in a gas or liquid of the type having transducer means for inducing elastic vibrations in a surface layer of the substrate and detector means for sensing changes in the elastic vibrations resulting from bonding of the substance to be detected with the surface layer, the improvement wherein

the surface layer (D'"'"') of said substrate (D) consists of a first substance having first active sites (A) adapted for covalent chemical bonding, coupling molecules (K) of a third substance each having at least one first active site adapted to form a covalent bond with a respective active site (A) of said first substance, and having at least one second active site adapted to form a second bond (C), and molecules (X) of a second substance, said molecules (X) having at least one first active site forming a bond with a second active site (C) of a respective coupling molecule (K), said molecules (X) having at least one second active site (B) in a point different from said first site said second active sites (B) of said molecules (X) being adapted for selective and reversible co-ordinative bonding with molecules (G) of the substance to be detected.

5. The substrate of claim 4, characterised in that said second bond is a substantially covalent bond.

6. The substrate of claim 4, characterised in that said second bond is a substantially coordinative bond.