Automatic analyzing apparatus for analyzing agglutination patterns

• US 4,861,554 A
• Filed: 04/08/1987
• Issued: 08/29/1989
• Est. Priority Date: 12/14/1981
• Status: Expired due to Term

• Current Assignee: Olympus Optical Corporation Limited (Olympus Corporation)
• Original Assignee: Olympus Optical Corporation Limited (Olympus Corporation)
• Inventors: Sakuma, Hajime
• Primary Examiner(s): Marcus, Michael S.
• Application Number:
  US07/036,210
• Time in Patent Office: 874 Days
• Field of Search: 422/63-67, 422/73, 422/102, 422/104, 435/291
• US Class Current: 422/65
• CPC Class Codes: G01N 2035/00752 bar codes
• CPC Class Codes: G01N 2035/0425 Stacks, magazines or elevators for plates
• CPC Class Codes: G01N 33/5302 Apparatus specially adapted for immunological test procedures
• CPC Class Codes: G01N 35/028 having reaction cells in the form of microtitration plates

Abstract

An automatic analyzing apparatus for analyzing agglutination patterns produced in response to an immunological agglutinating reaction is disclosed. The apparatus comprises a device for carrying a plurality of sample tubes which accommodate blood samples to be analyzed therein at a delivery position in order, a device for forming a plurality of diluent blood samples by diluting the blood samples in the tubes at the delivery position, and a device for transporting microplates having blood samples and reagents delivered in the reaction vessels along the line in a substantially stationary manner.

Claims

1. An immunological agglutination reaction analyzing apparatus comprising:

a microplate carrying line including, in sequence, a microplate feeding line, a reagent delivery transporting line, a microplate sending line, a reaction transporting line, a measurement transporting line and a microplate discharging line, said microplate sending line, said reaction transporting line and said measurement transporting line together forming a reaction line;

a plurality of sample tubes;

means for carrying said plurality of sample tubes along a sample carrying line to successively place said sample tubes at a first delivery position, said sample tubes containing blood samples to be analyzed, said means for carrying a plurality of sample tubes comprising;

a plurality of removable racks;

a cassette tray having a plurality of partitions successively arranged at spaced distances to define a plurality of gaps, each gap being constructed to receive a removable rack containing a plurality of sample tubes;

means for receiving said cassette tray and for intermittently advancing said cassette tray towards a discharge position, said cassette tray being advanced by the distance between adjacent partitions for each intermittent advance and presenting racks successively at a feed position;

means for moving a rack selected from a gap of a cassette tray located at said feed position after each intermittent advance and transporting that rack to position its sample tubes at said first delivery position; and

means for returning the selected rack to said gap of the cassette tray located at said feed position prior to the next advance;

means for forming a plurality of diluted blood samples by transferring a blood sample from each sample tube at the first delivery position to a respective diluting tube located at a beginning end of a diluted blood sample carrying line, and by adding diluent to each respective diluting tube, said means for forming a plurality of diluted blood samples comprising, a plurality of diluting tubes, means for delivering a blood sample contained in a sample tube into a plurality of diluting tubes and means for discharging diluent solutions into each respective diluting tube at the same time or before a blood sample is delivered therein from a sample tube to form diluted blood samples;

means for carrying the diluted blood samples within the diluting tubes along said diluted blood sample carrying line to a position adjacent a beginning end of said microplate sending line;

a plurality of microplates;

means for successively feeding said plurality of microplates to the beginning end of said reagent delivery transporting line from said microplate feeding line, each of said microplates including a plurality of reaction vessels therein, said microplate feeding means comprising;

means for holding a vertical stack of said plurality of microplates;

belt drive means having a belt for receiving the lowest microplate from the stack and a claw for engaging a rear surface of a received microplate; and

means engaging a channel in a side surface of a microplate for detecting a direction of orientation of the microplate;

first transporting means for transporting said microplates in a horizontal direction along said reagent delivery transporting line to a reagent delivery position at said beginning end of said microplate sending line;

means disposed adjacent said reagent delivery position for delivering given amounts of the diluted blood samples from the diluting tubes in said diluted blood sample carrying line into at least one reaction vessel of said microplates positioned at the beginning end of said microplate sending line;

means disposed adjacent said reagent delivery position for delivering given amounts of reagents into said at least one reaction vessel at the beginning end of said microplate sending line in accordance with an analysis to be performed;

first sending means for sending microplates with reaction vessels having said diluted blood samples and reagent contained therein in a horizontal direction along said microplate sending line to the beginning end of said reaction transporting line;

means for lowering said microplates with reaction vessels vertically in a stacked configuration along said reaction transporting line to the beginning end of said measurement transporting line in a substantially non-vibrating manner such that antigen and antibody combination reactions may occur in said at least one reaction vessel as a result of the receipt of said diluted blood sample and reagent in said at least one reaction vessel to form an agglutination pattern on a bottom surface of said reaction vessel, said means for lowering said microplates comprising,

first circulating belt drive means having a belt defining a drive axis which extends in the vertical direction and a plurality of partition members disposed on said belt orthogonal to said drive axis and equidistant from one another,

second circulating belt drive means having a belt defining a drive axis which extends in the vertical direction and a plurality of partition members disposed on said belt orthogonal to said drive axis and equidistant from one another, said first and second circulating belt drive means being positioned in opposed relation such that the vertical planes including the drive axis of each circulating belt drive means are parallel to one another and separated by a distance equal to the width of a microplate and such that each partition on one belt of a circulating drive means is in the same horizontal plane as a partition on the belt of the opposed circulating drive means along the length of the drive axis to form pairs of opposed partitions, and means for synchronously moving the belts of said circulating belt drive means to intermittently move said opposed partitions vertically while maintaining said horizontal plane, said circulating belt drive means being positioned such that microplates are received by said pairs of opposed partitions from said microplates sending line in a horizontal position and thereafter moved vertically;

second transporting means for transporting said at least one reaction vessel having agglutination patterns formed on a bottom surface thereof horizontally along said measurement transporting line to a measuring position;

means for photoelectrically detecting agglutination patterns formed on said bottom surface of said at least one reaction vessel at said measuring position to thereby produce a detection signal;

means for receiving said detection signal to effect an analysis as a result of a detected signal agglutination pattern;

second sending means for horizontally sending microplates after measurement to a raised position; and

means for discharging said microplates along said microplate discharging line from said raised position after visual observation of the agglutination patterns in all reaction vessels of one microplate.

2. The apparatus of claim 1 wherein said means for forming a plurality of diluted blood samples includes means for forming a blood cell suspension used for immunological agglutination reactions, said means for forming a blood cell suspension including means for delivering diluent solution and blood cell solutions to a diluting vessel in such a manner that the delivery of the diluent solution begins prior to or simultaneous with the delivery of the blood cell solution to the diluting vessel.

3. The apparatus of claim 1 wherein said fist transporting means and said second transporting means each includes belt drive means having a belt for transporting microplates between guide walls for intermittently advancing said groups of reaction vessels in a microplate by a predetermined distance and stopping means having a claw for engaging notches formed in a side of a microplate for surely stopping microplate movement after each advance.

4. The apparatus of claim 1 wherein said first sending means comprises:

a support member;

a branch member pivotally coupled to said support member and having a first arm engageable with a rear surface of a microplate and a second arm having a slidable member;

drive means for reciprocally moving the support member to linearly push microplates along guide walls of the reaction line when said first arm is enagaged with the rear surface of a microplate;

guide means for guiding said slidable member including a forward path guide surface and a backward path guide surface which engage said slidable member, said slidable member causing said first arm to engage said microplate during engagement of said slidable member with said forward path guide surface and causing said first arm to disengage said microplate during engagement of said slidable member with said backward path guide surface and means for causing said slidable member to change engagement from said forward path guide surface to said backward path guide surface; and

return means for moving said second arm such that the slidable member moves from engagement with said backward path guide surface to said forward path guide surface.

5. The apparatus of claim 1 wherein said means for discharging microplates comprises:

first drive means having a supporting arm for supporting a bottom surface of a microplate and driving the microplate in a vertical direction; and

second drive means having an engaging arm for engaging a front surface of a microplate and driving a microplate in a horizontal direction; and

wherein said means for discharging comprises;

means for pushing on the bottom surface of a microplate received from said second drive means to move said microplate in a vertical upward direction; and

detent means for receiving and holding a microplate pushed in said vertical upward direction.

6. The apparatus of claim 1 wherein said means for discharging microplates includes means for allowing visual observation of said microplates prior to discharge.