Apparatus for the detection of chemiluminescence

• US 4,863,689 A
• Filed: 11/30/1987
• Issued: 09/05/1989
• Est. Priority Date: 04/23/1987
• Status: Expired due to Fees

• Current Assignee: University of Victoria
• Original Assignee: University of Victoria
• Inventors: Fox, Glyn R., Leong, Merlin L., Hayward, John S.
• Primary Examiner(s): Shah, Mukund J.
• Application Number:
  US07/126,857
• Time in Patent Office: 645 Days
• Field of Search: 422/52, 422/61, 422/68, 422/102, 422/104, 422/66, 23/230 R, 23/230 B, 435/8, 435/35, 435/291, 436/135, 436/172, 436/546
• US Class Current: 422/52
• CPC Class Codes: C12Q 1/26 involving oxidoreductase
• CPC Class Codes: C12Q 1/66 involving luciferase
• CPC Class Codes: G01N 2021/1768 using photographic film
• CPC Class Codes: G01N 21/76 Chemiluminescence; Bioluminescence
• CPC Class Codes: G01N 21/763 Bioluminescence
• CPC Class Codes: G01N 2201/0648 Shutters
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10T 436/206664 Ozone or peroxide

Abstract

Apparatus is provided for the detection of chemiluminescence, e.g., the luminescent assay of an analyte in a sample. Sample, namely the production of a permanent photographic record of such chemiluminescence, e.g., by the use of a POLAROID film. The apparatus includes three interrelated elements. The first element comprises a film holder for holding a photographic film sensitive to a chemiluminescent reaction of the sample, the film holder defining a window in registry with any photographic film which may be held in the film holder. The second element comprises a sample holder for holding the sample in registry with the window and any photographic film which may be held in the film holder, and for allowing movements of the sample relative to any photographic film which may be held in the film holder between (i) a raised position, wherein the sample holder, and thus the sample is in spaced relation to any photographic film which may be held in the film holder and (ii) a lowered position, wherein said sample holder is in sufficiently close proximity to any photogarphic film which may be held in the film holder means to allow the sample held thereby directly to contact any photographic film which may be held in the film holder. The third element comprises a shutter operatively associated with the window of the film holder and movable between a closed position, wherein the shutter obstructs the window so as to prevent the sample holder from moving into the lowered position, and an opened position, wherein the window is unobstructed by the shutter so as to permit the sample holder to move from the raised position to the lowered position. The production of the permanent photographic record begins when the test sample in the container is placed in direct contact with the photographic film. The production of the photographic record is stopped when the test sample in the container is removed out of direct contact with the photographic film and the slidig shutter is closed. Various test results can be observed by varying the time of direct contact of the test sample in the reactant container with the photographic film. This provides maximal analytical sensitivity. During the entire test, exposure to ambient light is prevented.

Claims

1. Apparatus for the detection of a chemiluminescent reaction and for the production of a permanent photographic record of such chemiluminescent reaction, comprising:

(A) a first element comprising;

(a) a sample holder including clamp means to hold a test sample in a transparent container in selected positions;

and (b) holding means to hold said clamp means in a first position of said selected positions, where said transparent container containing said test sample is out of contact with said photographic film supply, and to release said clamp means to a second position of said selected positions, where said transparent container containing said test sample is in direct contact with said photographic film supply;

(B) a second element comprising;

(c) a supply of photographic film sensitive to chemiluminescence;

and (d) a shutter means to enable a source of chemiluminescence directly to contact a selected unit of said photographic film;

and (C) a third element comprising;

(e) an adaptor to provide a light-tight union between said first element (A) and said second element (B), said adaptor including a central window through which said sample holder projects;

whereby, upon manual actuation of said holding means to release said sample clamp means said sample holder is dropped, thereby to place said container containing said test sample into direct contact with said selected unit of photographic film.

2. The apparatus of claim 1 wherein said element (B) comprises a box holding a plurality of units of prepackaged, instant-developing photographic film.

3. The apparatus of claim 2 wherein said shutter means comprises a sliding shtter which is manually actuatable to provide a central open window to permit said holding means to drop said sample holder to place said container containing said test sample into direct contact with a selected unit of said photographic film.

4. The apparatus of claim 1 wherein said sample clamp comprises a box having an open bottom floor, and includes means to hold said test sample in said container therein.

5. The apparatus of claim 4 wherein said test sample in said container is in the form of a sample sheet absorbed with said test sample and protected within a transparent sample container.

6. The apparatus of claim 5 wherein said sample sheet comprises a nitrocellulose membrane having luminescent analyte absorbed thereon.

7. The apparatus of claim 4 wherein said test sample in said container is in the form of a plurality of well plates or tubes containing liquid luminescent analyte.

8. The apparatus of claim 4 wherein the inner face of said sample clamp means is provided with a light-reflective surface.

9. The apparatus of claim 4 wherein said sample clamp is held to said sample holder by a lockable position adjuster against the urging of spring means.

10. The apparatus of claim 9 wherein said spring means comprises a leaf spring.

11. The apparatus of claim 9 wherein said position adjuster is adapted to be unlocked to release said sample clamp means to allow said sample holder to be positively urged downwardly by said spring means.

12. The apparatus of claim 1 wherein said third element (C) is of the type including cooperating tongues-and-grooves, or hinges and clamps.

13. Apparatus for producing a permanent photographic record of a chemiluminescent reaction of a sample comprising:

film holder means for holding a photographic film sensitive to a chemiluminescent reaction of said sample, said film holder means defining a window in registry with any photographic film which may be held in said film holder means;

sample holding means for holding said sample in registry with said window and any photographic film which may be held in said film holder means, and for allowing movements of said sample relative to any photographic film which may be held in said film holder means between (i) a raised position, wherein said sample holding means, and thus said sample is in spaced relation to any photographic film which may be held in said film holder means and (ii) a lowered position, wherein said sample holding means is in sufficiently close proximity to any photographic film which may be held in said film holder means to allow said sample held thereby directly to contact any photographic film which may be held in said film holder means; and

shutter means operatively associated with said window of said film holder means and movable between a closed position, wherein said shutter means obstructs said window so as to prevent said sample holding means from moving into sad lowered position, and an opened position, wherein said window is unobstructed by said shutter means so as to permit said sample holding means to move from said raised position to said lowered position.

14. The apparatus of claim 13 including photographic film held in said film holder means.

15. The apparatus of claim 14 wherein said film is instant developing film.

16. The apparatus of claim 13, wherein said sample holding means includes sample clamp means for releasably holding said sample holding means in said raised position.

17. The apparatus of claim 16 wherein said sample clamp comprises a box having an open bottom floor, and includes means to hold said test sample in said container therein.

18. The apparatus of claim 13 wherein said test sample in said container is in the form of a sample sheet absorbed with said test sample and protected within a transparent sample container.

19. The apparatus of claim 18 wherein said sample sheet comprises a nitrocellulose membrane having luminescent analyte absorbed thereon.

20. The apparatus of claim 13 wherein said test sample in said container is in the form of a plurality of well plates or tubes containing liquid luminescent analyte.

21. The apparatus of claim 13 wherein the inner face of said sample clamp means is provided with a light-reflective surface.

22. The apparatus of claim 21 wherein said sample clamp is held to said sample holder by a lockable position adjuster against the urging of spring means.

23. The apparatus of claim 22 wherein said spring means comprises a leaf spring.

24. The apparatus of claim 22 wherein said position adjuster is adapted to be unlocked to release said sample clamp means to allow said sample holder to be positively urged downwardly by said spring means.

25. The apparatus of claim 13 including means providing a light-tight union between such film holder means and said sample holding means.